Considerable evidence has verified that microRNAs (miRNAs) play important roles in various cellular processes including differentiation. However, the regulatory roles of miRNAs involved in the differentiation of induced pluripotent stem cells (iPSC) into lung epithelial cells are still unknown. In this study, we first evaluated the current protocols to differentiate iPSC into alveolar epithelial type II (AEC II) cells, but the efficiency is low. We next identified that miR-22 can efficiently enhance the differentiation of iPSC into AEC II cells under the stimulation of proper growth factors and growing on appropriate matrix. Moreover, the AEC II cells generated from iPSC with miR-22 overexpression can proliferate and secrete lung surfactant. Here, we discovered a previously unknown interaction between miR-22 and iPSC differentiation but also provide a potential target for the effective derivation of AEC II from iPSCs for cell-based therapy.
Our study intends to assess the effect of transplantation of bone marrow mesenchymal stem cells (BMSCs) on nerve cell in rats with cerebral infarction and injury. 36 healthy rats were separated into JS group (sham-operation), NG group (cerebral infarction) and YZ group (BMSCs transplantation).
The arrangement of brain tissue in JS group was integrated without edema and confused in NG group with obvious edema. However, the necrosis degree of brain tissue in YZ group was alleviated. There were symptoms of muscle loss in the right foreleg and hind leg of rats in NG group. The NSS score
in NG group was higher than JS group (P < 0.05), suggesting that the neurological function after BMSCs transplantation was improved. The distribution of cortical tissue was tight and nervous tissue distribution in NG group was evacuated comparatively. The quantity of nerve cell was
reduced with tumescence. In conclusion, transplantation of BMSCs exerts a promising therapeutic effect on the treatment of cerebral infarction as it can improve nerve cell function, reduce oxidative stress and inflammatory cytokines secretion, and ameliorate cerebral injury.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.