Vitexin, the main flavonoid was extracted from the leaves of Combretum micranthum in soxhlet with ethanol. The extract evaporated to dryness and retrieved thanks to hot water. That liquid extract went through a degreasing and extracted again with butanol. The butanolic extract evaporated to dryness and dissolved in methanol, in which the vitexin precipitated after the washing and the recrystallisation of the first obtained precipitate. From 500g of dry kinkeliba leaves, a series of manipulations led to the isolation of 0.0576 g of vitexin, yielding 0.013% per ounce of the kinkeliba leaves dry powder. The degree of purity of vitexin was 94.79% on the basis of HPLC/UV-Vis/MS of the chemical formula C21H20O10. According to the spectral data NMR 1 H and 13 C the molecular structure of the vitexin is identified.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.