Abstract. Biogas will be produced from a single organic substrate and controlled the ratio of carbon nitrogen by added inorganic nitrogen substances. The study has been tried to combine substrates from industrial tapioca industrial wastewater with tofu industrial wastewater. The experiments were carried out in batch anaerobic digesters with a C/N ratio range of 20 -30 and variation of microbial sludge from cow rumen, anaerobic sludge of tofu wastewater treatment plant (WWTP) and anaerobic sludge of tapioca WWTP. The ratio of C/N in the range of 20-30 didn't significant effect on the volume of biogas production. The microbial from cow rumen gives the best biogas production performance. Biogas yield is 2,199 liter per kg COD.
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