Libo leaves (Ficus variegate Blume) is never eaten by caterpillars. This is indicated that libo leaves has a good secondary metabolites. This research was conducted on optimization of extraction techniques and test of secondary metabolites of leaves Libo. The optimization was done by changing variables viz. method of extraction, variation of amount of solvent, and duration of extraction. The effectiveness of extraction was seen from % of yield. Method of extraction was using maceration and soxhlet. Methanol was used as
Research about chromatographic profile of antioxidant and antibacterial compounds in nhexane fraction of Libo leaves (Ficus variegata Blume.)
Key Words: n-hexane fraction of Libo leaves (Ficus variegata Blume.), chromatography, antioxidant, antibacterial
ABSTRAKPenelitian mengenai profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi nheksana dari daun Libo (Ficus variegata Blume.) telah dilakukan dengan tujuan untuk mengetahui profil kromatografi senyawa antioksidan dan antibakteri dalam fraksi n-heksana dari daun Libo. Ekstraksi dilakukan secara maserasi dengan menggunakan pelarut metanol. Isolasi dilakukan dengan 2 metode, kromatografi kolom vakum (KCV) kemudian dilanjutkan dengan kromatografi kolom konvensional (KK). Eluen yang digunakan yaitu n-heksan-etil asetat dan metanol-kloroform. Hasil pemisahan yang diperoleh berdasarkan kromatografi kolom konvensional yaitu sebanyak 10 fraksi. Fraksi-fraksi tersebut kemudian dilakukan pengujian aktivitas antioksidan secara kualitatif menggunakan metode DPPH serta pengujian aktivitas antibakteri menggunakan metode KLT bioautografi. Hasil penelitian yang diperoleh berupa profil kromatografi fraksi yang memiliki aktivitas antioksidan dan antibakteri, yaitu sebanyak 5 fraksi (NHB1, NHB3, NHB4, NHC1, NHC3) aktif sebagai antioksidan dan 4
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