This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.
In order to enhance natural products value, Melissa officinalis (lemon balm) aerial part (leaves) has been studied in this work. Hence, the objective of this study is to determine the chemical composition of the studied plant polyphenols extracts using HPLC/DAD, as well as evaluate their flavonoid extracts’ antioxidant and antimicrobial activities using DPPH• and disk diffusion methods, respectively. The results of phenols chemical composition showed the existence of two phenolic acids, five flavonic aglycones and six heterosides, while the biologic results of the plant flavonoid extracts exhibited the existence of a good antioxidant and antimicrobial activities.
The leaves of Thymus algeriensis Boiss. collected from middle-North of Algeria (Sidi Aissa, M'sila). Essential oil from the stem bark of Algerian species of Thymus algeriensis Boiss. of the family of Lamiaceae was obtained by hydro-distillation using Clavenger apparatus, possessed an essential oil in 1.3 % (v/w) yield. GC and GC/MS analysis were carried out on the essential oil and was found to contain Seventy-one compounds, representing 95.99 % (area percent) of the total oil composition. Oil was rich in Oxygenated Monoterpenes (45.14 %), exhibited higher percentage of Camphor (22.60 %) followed by Camphene (12.78 %), Borneol (11.16 %), 1,8-Cineole (5.94 %), Acorenone (5.84 %) and α-Pinene (5.01 %). The principal constituents are hydrocarbons, ketones, alcohols, esters and aldehydes. The antioxidant activity of essential oil was investigated by measuring the decrease in absorption at 517 nm of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in a UV/visible spectrophotometer. The oil exhibited weak antioxidant activity by DPPH free radical scavenging bioassay (IC 50 = 83.8 mg/ml).
The chemical composition was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The constituents were identified by their mass spectra and Kovats' indices. Fivety (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were : 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97 %), Germacrene D-4-ol (9.61 %), α-pinéne (9.37 %), Phytol (4.87 %), Dehydro-sabina ketone (4.12 %), Piperitone (3.27 %), δ-Cadinene (3.13 %), 1-Octen-3-ol (2.35 %) and Benzaldehyde (2.31 %). The antioxidant activity of the oils was assessed by measurement of metal chelating activity, the reductive potential, the free radical scavenging (DPPH) assay. The antioxidant activity was compared with that of synthetic antioxidants: butylated hydroxyanisole (BHT) , and the essential oils . The results showed as the Essential oil of M vulgare was evaluated for its antibacterial activities against Gram-positive and Gram negative pathogenic bacteria: Listeria monocytogene, Pseudomonas aeruginosa , Agrobacterium tumefaciens and Salmonella enterica.
The leaves of Thymus algeriensis Boiss. collected from middle-North of Algeria (Sidi Aissa, M'sila). Essential oil from the stem bark of Algerian species of Thymus algeriensis Boiss. of the family of Lamiaceae was obtained by hydro-distillation using Clavenger apparatus, possessed an essential oil in 1.3 % (v/w) yield. GC and GC/MS analysis were carried out on the essential oil and was found to contain Seventy-one compounds, representing 95.99 % (area percent) of the total oil composition. Oil was rich in Oxygenated Monoterpenes (45.14 %), exhibited higher percentage of Camphor (22.60 %) followed by Camphene (12.78 %), Borneol (11.16 %), 1,8-Cineole (5.94 %), Acorenone (5.84 %) and α-Pinene (5.01 %). The principal constituents are hydrocarbons, ketones, alcohols, esters and aldehydes. The antioxidant activity of essential oil was investigated by measuring the decrease in absorption at 517 nm of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in a UV/visible spectrophotometer. The oil exhibited weak antioxidant activity by DPPH free radical scavenging bioassay (IC50 = 83.8 mg/ml).
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