Background Coronavirus-2019 (COVID-2019) is a novel coronavirus known as Acute Respiratory Syndrome (SARS-CoV-2). The premier standard test for SARS-CoV-2 diagnosis is a one-step RT-qPCR method, which requires specific probes and reagents. Therefore, detection on a large scale is expensive and cannot be very accurate. Methods A cost-effective technique based on SYBR green was evaluated in the current study. The specific primers for S and N genes were designed, then performed the cross-reactivity test with other coronavirus and respiratory viruses positive samples. Moreover, the analytical sensitivity test was carried out with 8 dilutions (1:10). Lastly, the SARS-CoV-2 clinical samples (n = 210) were tested by these two methods, and receiver operating characteristic (ROC) analysis was performed to investigate the incremental diagnostic value of each gene in the study methods. Results The two-step method detected up to 6th dilutions of the SARS-CoV-2 samples and did not show any amplification of the positive samples of other respiratory viruses. ROC analysis revealed a diagnostic ability of the two-step method for SARS-CoV-2 with an area under the ROC curve of ≥ 0.7 (P ˂ 0.05) and relatively high sensitivity and specificity. The combination of N and S genes increased the sensitivity up to 88%, specificity up to 86%, and area under the ROC curve up to 0.85 (95% confidence interval (95% CI) 0.72 to 0.93, P = 0.0461). Conclusion Our findings indicated that the two-step method has comparable sensitivity and specificity to the one-step method. Therefore, this method can be considered a potential diagnostic method for diagnosing and monitoring COVID-19 patients. It suggests that when the one-step RT-qPCR method is not available, the two-step RT-qPCR can be used to identify SARS-CoV-2.
Background: In the 21st century, the world has been facing several emerging viral epidemics. These viruses are divided into two categories, including emerging viruses and re-emerging viruses. Factors, such as climate change, global warming, and some eating habits will have irreversible effects on human, animal, and environmental ecosystems. These emerging or re-emerging infectious diseases have significant effects on the world economy and health. The peak of this type of infectious disease appeared in the 1980s with a viral epidemic called human immunodeficiency viruses (HIV). These emerging or recurring viruses have affected both livestock and humans. There is evidence that these emerging or re-emerging viruses, influenced by the environment and the human diet are also affected by climatic cultures. Therefore, it would be possible to identify the center areas for the emergence or re-emergence of these viral diseases. In this review article, it has been attempted to identify world resources and the way we should be prepared for a confrontation with these deadly viruses through the study of these epidemics, emerging, and re-emerging viruses, since the mutated viral infections may emerge and re-emerge in the near future. For this review study, the keywords, including COVID-19, Coronavirus, SARS-COV2, Influenza virus (H1N1), Zika virus, HIV1 virus, Hanta virus, Ebola, Influenza (H5N1), Nipah, Hendra, West Nile fever, SARS- COV1, MERS-CoV were collected in PubMed, Scopus, Google Scholar, and other reputable databases.
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