Amaç: Endemik Ferula tenuissima köklerinin fitokimyasal yönden incelenmesi ve elde edilen saf bileşiklerin PC-3 üzerinde sitotoksik etkilerinin belirlenmesidir. Gereç ve Yöntemler: F. tenuissima 'nın açık havada kurutulmuş ve toz haline getirilmiş kökleri (1 kg, 24 saat boyunca 30°C'de sonikasyonda n-hexane, kloroform (CHCl 3) ve metanol (MeOH) (3×2 L, her biri) ile sırasıyla ekstre edilmiştir. Ekstraktlar daha sonra süzülmüştür. Sıvı ekstreler, vakum altında kuruluğa kadar uçurulmuştur. Kromatografik yöntemler ile bileşikler elde edilmiş ve bileşiklerin yapıları spektral yöntemler ile belirlinmiştir (1D-, 2D NMR ve LC-MS). Bileşiklerin PC-3 üzerindeki sitotoksik aktiviteleri WST yöntemi ile test edilmiştir. Bulgular: Endemik F. tenuissima'nın kurutulmuş köklerinin fitokimyasal incelemesi yapılmış ve üç seskiterpen esteri izole edilmiştir. Teferidin, ferutinin ve elaeochytrin-A daukan tip seskiterpen esterleri olarak belirlenmiştir. Biyoaktivite çalışmasında, ferutinin en yüksek sitotoksik aktiviteyi IC 50 : 19.7 µM ile göstermiştir. Sonuç: F. tenuissima köklerinin ana bileşiklerinin daukan seskiterpenler ve ferutininin PC-3 hücreleri üzerinde potansiyel bir etkiye sahip olduğunu göstermektedir. Anahtar kelimeler: Ferula tenuissima, daukan seskiterpen ester, sitotoksite, prostat kanseri Objectives: This was a phytochemical study of endemic Ferula tenuissima roots and determined the cytotoxic activity of pure compounds on PC-3. Materials and Methods: Air-dried and powdered roots of F. tenuissima (1 kg) were extracted consecutively with n-hexane, chloroform (CHCl 3), and methanol (MeOH) (3×2 L, each) by sonication at 30°C for 24 h. The extracts were then filtered. The solvents were separately evaporated under reduced pressure to dryness. The compounds were isolated by chromatographic methods and their structures were determined by spectral methods (1D and 2D NMR and LC-MS). The compounds were tested for their cytotoxic activities versus the PC-3 cell line by WST assay. Results: A phytochemical investigation of the dried roots of endemic F. tenuissima was performed and three sesquiterpene esters were isolated. The daucane-type sesquiterpenes teferidin, ferutinin, and elaeochytrin-A were identified. In the bioactivity study, ferutinin exhibited the highest cytotoxic activity, with an IC 50 value of 19.7 µM. Conclusion: The results indicate that the main compounds of F. tenuissima roots are daucane sesquiterpenes and ferutinin has a potential effect on PC-3 cells.
Ferula L. is represented by 23 taxa in Turkish flora. Most of the species are known as "Caksır" and roots are used as aphrodisiac in Anatolia. The aim of this study is to investigate the cytotoxic potential of roots of twelve different Ferula L. species distributing in Anatolia on prostate cancer cell line PC-3. Three extracts with different polarity were prepared from grounded roots of plants by ultrasonic solvent extraction. WST assay was performed to evaluate the cytotoxic effects of extracts. High cytotoxic activity was observed in F. elaeochytris, F. drudeana chloroform extracts and F. szowitsiana hexane extract (IC50: 3.21±0.24µg/ml, 5.92±0.53µg/ml, 8.03±0.51µg/ml respectively) whereas methanol extracts of all species had no cytotoxic activity. F. tingitana chloroform extract induced high proliferation in dose dependent manner. A daucane ester "ferutinin" content was evaluated among the extracts by UPLC and it is found in highest concentrations in F. tenuissima and F. halophila hexane extracts (167380 µg/g and 157160 µg/g respectively). Although there is not a direct relationship between ferutinin content and cytotoxicity on PC-3, F. elaeochytris, F. drudeana, F. szowitsiana and F. tenuissima can be good candidates for the further studies for finding lead phytochemical compounds for prostate cancer treatment.
Objectives: Phytochemical study of endemic Ferula tenuissima roots and determination of the cytotoxic activity of pure compounds on PC-3. Materials and Methods: Air-dried and powdered roots of F. tenuissima (1 kg) were extracted respectively with n-hexane, chloroform (CHCl3) and methanol (MeOH) (3x2 L, each) by sonication at 30 ºC, for 24h. The extracts were then filtered. The solvents separately evaporated under reduced pressure to dryness. Compounds were isolated by chromatographic methodes and structures of compounds were determined by spectral methodes (1D-, 2D NMR and LC-MS). Compounds were tested for their cytotoxic activities versu PC-3 cell line by WST assay. Results: Phytochemical investigation of dried roots of endemic F. tenuissima performed and three sesquiterpene esters were isolated. As teferidin, ferutinin and elaocyhtrin-A; daucane-type sesquiterpenes were identified. In the bioactivity study, Ferutinin exhibitited the highest cytotoxic activity with 19.7 µM IC50 value. Conclusion: The results indicate that the main compounds of Ferula tenuissima roots are daucane sesquiterpenes and ferrutinin has potential effect on PC-3 cells.
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