HIV (human immunodeficiency virus) belong to family of human retroviruses of lentivirus subfamily. This virus hits the patient’s immune system by destroying important cells that fight against disease and infection. Different parameters like Total Leucocytes count TLC, Hb level, platelets count and among male and female were observed.Study of different parameters in HIV (human immunodeficiency virus) patientsfrom different areas of Punjab, Pakistan. Complete blood count (CBC), Hb Level, HIV (human immunodeficiency virus), Platelets count, Total Leucocytes count TLC was done using kits and screening methods.RNA extraction and PCR was done for future analysis.HIV positive males develop anemia and about 9.3% (11) patients are suffering from leukocytosis. 12% of HIV positive males develop thrombocytopenia while 8% have thrombocytosis.

Hematological and hematopoietic cells malignancies of the genes and hematopoietic cells are associated with the genetic mutation, often at the chromosomal level. The standard cytogenetic study is widely accepted as one of the main diagnostics and prognostic determinants in patients. Therefore, the current descriptive and cross-sectional study sought to determine the cytogenetic analysis of frequent hematological malignancies in Pakistan. A total of 202 peripheral bone marrow or blood samples from patients with benign and malignant hematological malignancy were taken using a conventional G-banding technique. Among enrolled patients, the mean age was 21.5 years ± 23.4, and gender-wise distribution showed a marked predominance of the male 147 (73%) population compared to the female 55 (27%). Patients in the age group (2-10 years) had the highest frequency, 48 (24%), of hematological neoplasms, followed by age (11-20 years) with 40 (20%). Normal karyotypes (46, XX/46, XY) was found in 51% (n=103) patients. Furthermore, the frequency of complex karyotype was 30 (15%), while normal was seen in 171 (85%) patients. Pre-B Acute Lymphoblastic Leukemia (Pre-B ALL) was the most prevalent malignancy of 66 (33%), followed by Chronic Myelogenous Leukemia (CML) of 41 (20%) and Acute Lymphocytic Leukemia of 29 (14%). Translocation was the most prevalent 50 (25%), followed by hypotriploidy 14 (7%) and monosomy 8 (4%) on chromosome aberration analysis. In addition, t(9:22) translocation was found to be 20 (10%) in CML, with the majority in the age group (31-40 years). This study recommends that karyotyping should be tested frequently in hematological conditions because it may provide insight into the relative chromosomal changes associated with particular malignancies.

RT-PCR is a gold standard test for the diagnosis of SARS-CoV2 (Covid-19) infection; however, it is an expensive, time consuming and technical demanding technique. Rapid antigen detection immunoassay (RAD) is cost-effective, quick as well as can be performed and interpreted easily. The rapid diagnosis of COVID-19 patients is essential to reduce cost and control the disease spread; however, the real world data of these tests must be validated with RT-PCR before they can be used at large scale. The objective of this study was to determine the sensitivity and specificity of PanbioTMCOVID-19 Ag-Rapid test device (Abbot) with multiplex RT-PCR. METHODS:  A total of n=3509 samples were tested for SARS-CoV-2 RAD and RT-PCR at Institute of Biomedical and Genetic Engineering, Islamabad. The rapid antigen tests were performed by PanbioTMCOVID-19 Ag-Rapid test device (Abbott) and compared with RT-PCR performed on Thermo Fisher (ABI) Quant Studio 5 using CDC 2019-nCoV RT-PCR protocol.  RESULTS: Total (n=3509), n=458 (7.60%) samples were reported positive by rapid antigen out of which n= 445 RT-PCR positive (13 false positive by rapid antigen), n=3051 (92.4%) were negative. True antigen negative tests n= 3051) were repeated with RT-PCR among these, n=25 were observed RT-PCR positive (rapid antigen false negative). The threshold cycle (CT) for the RT-PCR tests of these samples was >30. CONCLUSION: PanbioTMCOVID-19 Ag-Rapid test devices (Abbott) showed a sensitivity ratio 94.6% compared to RT-PCR. The PanbioTMCOVID-19 Ag-Rapid test device (Abbott) is reliable and can be used for screening and isolation of positive patients from the population.