Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is soil-borne disease of banana (Musa spp.) causing significant economic losses. Extensins and arabinogalactan proteins (AGPs) are cell wall components important for pathogen defence. Their significance for Foc resistance in banana was not reported so far. In this study, two banana cultivars differing in Foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and AGPs after wounding and Foc inoculation. Extensins mainly appeared in the root cap and meristematic cells. AGPs recognized by JIM13, JIM8, PN16.4B4 and CCRC-M134 antibodies located in root hairs, xylem and root cap. Individual AGPs and extensins showed specific radial distribution in banana roots. At the transcript level, seven extensins and 23 AGPs were differentially expressed between two banana cultivars before and after treatments. Two extensins and five AGPs responded to the treatments at the protein level. Most extensins and AGPs were up-regulated by wounding and pathogen inoculation of intact plants but down-regulated by pathogen attack of wounded plants. Main components responsible for the resistance of banana were MaELP-2 and MaPELP-2. Our data revealed that AGPs and extensins represent dynamic cell wall components involved in wounding and Foc resistance.
BackgroundCysteine-rich peptides (CRPs) are gaining recognition as regulators of cell–cell communication in plants.ResultsWe identified 9556 CRPs in 12 plant species and analysed their evolutionary patterns. In most angiosperm plants, whole genome duplication and segmental duplication are the major factors driving the expansion of CRP family member genes, especially signal peptides. About 30% of the CRP genes were found clustered on the chromosomes, except in maize (Zea mays). Considerable collinearities between CRP genes between or within species reveal several syntenic regions on the chromosomes. Different subfamilies display diverse evolutionary rates, suggesting that these subfamilies are subjected to different selective pressures. CRPs in different duplication models also show contrasting evolutionary rates, although the underlying mechanism is unclear because of the complexity of gene evolution. The 1281 positively selected genes identified are probably generated within a certain period of time. While most of these belonged to maize and sorghum (Sorghum bicolor), new CRP functions would also be expected. Up-regulation of 10 CRPs was observed in self-pollinated pear pistils and pollen tubes under self S-RNase treatments in vitro. The expression divergence between different CRP gene duplication types suggests that different duplication mechanisms affected the fate of the duplicated CRPs.ConclusionOur analyses of the evolution of the CRP gene family provides a unique view of the evolution of this large gene family.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3948-3) contains supplementary material, which is available to authorized users.
The cultivation and the usage of maize have been significantly increased across all provinces of Rwanda. Nevertheless, the problem of aflatoxin contamination remains a major factor that renders them to be unfit for animal and human consumption. In this research, the effects of drying methods (sun drying, kitchen drying, dry shelter) post aflatoxin infection in maize were evaluated in Northern Rwanda. A randomized complete block design with four replications was used with maize variety (H628) with drying methods as the main plot. Maize samples were tested for aflatoxin using aflatest. In both seasons (2021 A and 2021 B) the aflatoxin results show that the aflatoxin infection levels were lower in maize dried with kitchen drying (1.4 ppb) compared to the samples dried with sun drying (1.6 ppb) and dry shelter (2.2). Also, it was seen that the three drying methods are good for reducing the aflatoxin infection levels lower than 10 ppb as standards limit for East African countries. The aflatoxin infection levels were found to be significant (≤10 ppb) as results of kitchen drying than sun drying and dry shelter methods. This research resulted that proper drying such as drying maize on kitchen fire (kitchen drying) produce the lower level of aflatoxin infection in maize.
The research was conducted in the year of 2021-2022 in Burera and Musanze District in Northern province as well as Rubavu District in Western Province with the aim of assessing the yield loss in common beans due to anthracnose by using four cultivars with various levels of resistance in field conditions in environment of Northwestern of Rwanda. Randomized complete block design as experiment design was used in this study where each selected cultivar was grown and the yield loss caused by anthracnose was assessed. A level of significance of P<0.05 . In field trials, on the particularly susceptible cultivar Gikundiro 2 particulary showed the maximum harm in terms of incidence and severity in both infection circumstances. The severity of pod infection ranging from 3-9 (0-) resulted in reduction in quantity of seeds which contain a pod ranging from 10.52 % -57.76 % and the loss in weight of seeds per pods ranging from 21.93 % - 68.77 %, both demonstrated a direct impact on seed yield. However, determinate cultivars showed a greater drop in yield (58.5%) than indeterminate cultivars (10.52%) although both were sensitive, based on this research, anthracnose causes economic yield loss on variety Gikundiro 2 and Vuzimpundu.
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