Understanding the regeneration process in an old growth forest is essential for restoration projects. The present research sought to characterize the floristic and structural composition of shrub-tree species in São Francisco de Assis Municipal Park in Blumenau-SC. We sampled 50 permanent plots divided into three height classes. We estimated the total natural regeneration index for each species, the Shannon-Wiener diversity index and Pielou equability, and classified the species according to life-form and ecological group. A total of 1048 individuals were found, distributed in 112 species, 69 genera and 37 families. The species with the highest values of total natural regeneration index were Rudgea recurva, R. jasminoides, Ouratea parviflora, Psychotria nuda and Virola bicuhyba. The Shannon and Pielou indices demonstrated high diversity and equability for the natural regeneration area sampled. The results indicate species possibly capable of reaching the tree strata, and also indicate high forest conservation.
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