Honeybees rely on nectar as their main source of carbohydrates. Sucrose, glucose and fructose are the main components of plant nectars. Intriguingly, honeybees express only three putative sugar receptors (AmGr1, AmGr2 and AmGr3), which is in stark contrast to many other insects and vertebrates. The sugar receptors are only partially characterized. AmGr1 detects different sugars including sucrose and glucose. AmGr2 is assumed to act as a co-receptor only, while AmGr3 is assumedly a fructose receptor. We show that honeybee gustatory receptor AmGr3 is highly specialized for fructose perception when expressed in Xenopus oocytes. When we introduced nonsense mutations to the respective AmGr3 gene using CRISPR/Cas9 in eggs of female workers, the resulting mutants displayed almost a complete loss of responsiveness to fructose. In contrast, responses to sucrose were normal. Nonsense mutations introduced by CRISPR/Cas9 in honeybees can thus induce a measurable behavioural change and serve to characterize the function of taste receptors in vivo. CRISPR/Cas9 is an excellent novel tool for characterizing honeybee taste receptors in vivo. Biophysical receptor characterisation in Xenopus oocytes and nonsense mutation of AmGr3 in honeybees unequivocally demonstrate that this receptor is highly specific for fructose.
Honeybees (Apis mellifera) need their fine sense of taste to evaluate nectar and pollen sources. Gustatory receptors (Grs) translate taste signals into electrical responses. In vivo experiments have demonstrated collective responses of the whole Gr-set. We here disentangle the contributions of all three honeybee sugar receptors (AmGr1-3), combining CRISPR/Cas9 mediated genetic knock-out, electrophysiology and behaviour. We show an expanded sugar spectrum of the AmGr1 receptor. Mutants lacking AmGr1 have a reduced response to sucrose and glucose but not to fructose. AmGr2 solely acts as co-receptor of AmGr1 but not of AmGr3, as we show by electrophysiology and using bimolecular fluorescence complementation. Our results show for the first time that AmGr2 is indeed a functional receptor on its own. Intriguingly, AmGr2 mutants still display a wildtype-like sugar taste. AmGr3 is a specific fructose receptor and is not modulated by a co-receptor. Eliminating AmGr3 while preserving AmGr1 and AmGr2 abolishes the perception of fructose but not of sucrose. Our comprehensive study on the functions of AmGr1, AmGr2 and AmGr3 in honeybees is the first to combine investigations on sugar perception at the receptor level and simultaneously in vivo. We show that honeybees rely on two gustatory receptors to sense all relevant sugars.
8Background: Honeybees rely on nectar as their main source of carbohydrates [1]. Sucrose, glucose 9 and fructose are the main components of plant nectars [2] [3]. Intriguingly, honeybees express only three 10 putative sugar receptors (AmGr1, AmGr2 and AmGr3) [4] , which is in stark contrast to many other 11 insects and vertebrates. The sugar receptors are only partially characterized [5] [6]. AmGr1 detects 12 different sugars including sucrose and glucose. AmGr2 is assumed to act as a co-receptor only, while 13 AmGr3 is assumedly a fructose receptor. 14 Results: We show that honeybee gustatory receptor AmGr3 is highly specialized for fructose perception 15 when expressed in Xenopus oocytes. When we introduced nonsense mutations to the respective AmGr3 16 gene using CRISPR/Cas9 in eggs of female workers, the resulting mutants displayed almost a complete 17 loss of responsiveness to fructose. In contrast, responses to sucrose were normal. Nonsense mutations 18 introduced by CRISPR/Cas9 in honeybees can thus induce a measurable behavioural change and serve 19 to characterize the function of taste receptors in vivo.20 Conclusion: CRISPR/Cas9 is an excellent novel tool for characterizing honeybee taste receptors in 21 vivo. Biophysical receptor characterisation in Xenopus oocytes and nonsense mutation of AmGr3 in 22 honeybees unequivocally demonstrate that this receptor is highly specific for fructose. Graphical Abstract 24 See Fig. 0. 25 Background 29 Honeybees (Apis mellifera) are not only important pollinators world-wide. The highly social insects 30 perform an intricate division of labour and are well-known for their astonishing skills in learning and 31 communication. When it comes to taste, however, honeybees display a rather poor set of receptors. 32Because plant-derived nectar is their sole source of carbohydrates, sugar perception is naturally of 33 utmost importance for honeybees. The bees sense the sugar composition of a food source with only a 34 few fine contact chemoreceptors on their antennal tip [7]. In contrast to many other insects such as the 35 fruit fly (Drosophila melanogaster) with 68 genes and mosquitoes (Anopheles gambiae) with 75 genes, 36 the genome of the honeybee comprises only ten genes coding for gustatory receptors (Grs) [4]. Among 37 these only three code for sugar receptors: AmGr1, AmGr2 and AmGr3 [4] [8]. The taste receptors are 38 expressed in the brain, the antennae, mouthparts, tarsi and the gut of the honeybee. With this small set 39 of receptors honeybees evaluate a diverse set of sugars like sucrose, fructose, maltose and melicitose 40 in nectar in varying composition and in amounts ranging from 5% to 80%. In flowers of mint plants 41 (Laminacea), buttercups and clematis (Ranunculaceae), for example, sucrose is the main sugar, 42whereas other flowers such as those of oilseed rape contain relatively more glucose and fructose [3] [9]. 43The sugar trehalose, in contrast, acts as blood sugar [10] [2]. 44How honeybees recognize the different sugars in nectar and in the inner organs ...
Honeybees (Apis mellifera) need their fine sense of taste to evaluate nectar and pollen sources. Gustatory receptors (Grs) translate taste signals into electrical responses. In vivo experiments primarily demonstrate collective responses of the whole Gr-set, but little is known about the individual impact of receptors. Here, we disentangle for the first time the contributions of three gustatory receptors (AmGr1-3) in sugar sensing of honeybees by combining CRISPR/Cas9 mediated genetic knock-out, electrophysiology and behaviour. AmGr1 responds to multiple sugars. Bees lacking this receptor have a reduced response to sucrose and glucose but not to fructose. AmGr2 acts as co-receptor of AmGr1 in a heterologous expression system, but honeybee knock-out mutants perform normally. Eliminating AmGr3 while preserving AmGr1 and AmGr2 abolishes the perception of fructose but not of sucrose. We thus dissociate the roles of AmGr1, AmGr2 and AmGr3 in honeybee taste perception.
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