Trichoderma harzianum isolates have been broadly used for biocontrol of plant diseases caused by fungi. Cladosporium herbarum is a common saprophyte and seed borne fungus, which is easy to manipulate under controlled conditions. It was chosen as a model to test the effectivity of seed treatments with T. harzianum. Common bean seeds (cv. Pérola) contaminated with C. herbarum were treated with conidial suspension (CS) and autoclaved filtrate (AF) of five isolates of T. harzianum and subsequently submitted to health and germination tests. The proportion of normal seedlings formed, the length of roots, hypocotyls and leaves, and total plantlet length, total plantlet biomass, root mass ratio (RMR), stem mass ratio (SMR), leaf mass ratio (LMR), aerial part/root system ratio (AP/RS) and leaf area were also evaluated. Isolates CEN289 and CEN290 (CS and AF) provided 66 to 77% of supression of C. herbarum on seeds and a higher number of normal seedlings as compared with control. It also yielded a higher total biomass of plantlets. Moreover treatment with isolates CEN289 and CEN290 increased root and stem length in the experiments with CS. Such results indicate the potential of T. harzianum for seed treatment and suggest that it should be further tested as control for seed borne fungal diseases and as a plant growth promoter. The better performance found for CEN289 and CEN290 confirms the variability in terms of biocontrol activity among strains of T. harzianum.
de agosto a setembro de 2012. O objetivo foi avaliar o desenvolvimento de quatro cultivares de alface em sucessão a leguminosas. O delineamento experimental foi o de blocos casualizados, com cinco repetições. A avaliação da produção foi pelo número de folhas e peso fresco de seis plantas úteis por tratamento, totalizando 30 plantas por tratamento. O plantio de alface, sucedendo o cultivo de leguminosas, proporcionou melhor desenvolvimento às cultivares Delícia, Grandes Lagos e Manteiga, as quais mais se destacaram em quantidade de folhas e peso fresco. Palavras-chaves: Adubação verde; Cultivo orgânico; Conservação do solo.This study was conducted at the State University of Goiás, UnU Ipameri, August-September 2012. The objective was to evaluate the development of four lettuce cultivars grown after legumes. The experimental design was a randomized block with five replications. The evaluation of the production was the number of leaves and fresh weight of six plants per treatment, totaling 30 plants per treatment. The planting of succeeding lettuce crop legume cultivars to enhanced growth Delight, Great Lakes and Butter, which stood out in number of leaves and fresh weight.
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