To date, there is a lack of research into the vaginal and sperm microbiome and its bearing on artificial insemination (AI) success in the ovine species. Using hypervariable regions V3–V4 of the 16S rRNA, we describe, for the first time, the combined effect of the ovine microbiome of both females (50 ewes belonging to five herds) and males (five AI rams from an AI center) on AI outcome. Differences in microbiota abundance between pregnant and non-pregnant ewes and between ewes carrying progesterone-releasing intravaginal devices (PRID) with or without antibiotic were tested at different taxonomic levels. The antibiotic treatment applied with the PRID only altered Streptobacillus genus abundance, which was significantly lower in ewes carrying PRID with antibiotic. Mageebacillus, Histophilus, Actinobacilllus and Sneathia genera were significantly less abundant in pregnant ewes. In addition, these genera were more abundant in two farms with higher AI failure. Species of these genera such as Actinobacillus seminis and Histophilus somni have been associated with reproductive disorders in the ovine species. These genera were not present in the sperm samples of AI rams, but were found in the foreskin samples of rams belonging to herd 2 (with high AI failure rate) indicating that their presence in ewes’ vagina could be due to prior transmission by natural mating with rams reared in the herd.
Zusammenfassung
Sperma‐Analyse bei Sichelzellerkrankung
Die Reproduktionsfunktion des Mannes bei Sichelzellerkrankung ist bisher kaum unter‐sucht worden. Niedrige Spermatozoenwerte, eingeschränkte Spermatozoenmotilität und Stillstand der Spermatogenese — nachgewiesen durch Hodenbiopsie — sind hierbei beschrieben worden.
In dem vorliegenden Patientenkollektiv wurden hauptsächlich folgende Veränderungen im Sperma gefunden: verringerte Vorwärtsbewegung der Spermatozoen und aufgrund der morphologischen Beurteilung eine hohe Inzidenz des Stressmusters (tapering — Formen der Spermatozoen). Da diese Ergebnisse den Befunden sehr ähnlich sind, die man bei einer Varicozele findet, wird von den Autoren eine Hypoxie des Hodens als möglicher Stressfaktor sowohl für Patienten mit einer Sichelzellerkrankung als auch mit einer Varicozele angesehen.
RH studies and 10 other men for the study of prolactin secretion after stimulation with sulpiride .A total of fifty-five patients with varicocele and infertility attending to our Andrologi-From this group, 23 men served as control for hormone basal level studies, 10 for LH-
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