The aim of the study was to evaluate the effect of feeding hempseed expellers in a feed mixture on the quality indicators of broiler chicken's meat. One hundred and fifty Ross 308 hybrid cockerels were used in the present study. The control group (HS0) was fed without hempseed expellers; the other two groups received diets containing 50 g·kg -1 and 150 g·kg -1 of hempseed expellers (HS5 and HS15, respectively). The birds were slaughtered at the age of 37 days, and samples of breast and thigh muscles were collected for determination of proximate chemical composition and technological properties, and sensory analyses. Feeding with hempseed expellers influenced the colour of meat with a significant difference observed for a* (redness) and b* (yellowness) values in the HS15 group. The colour of breast meat in HS15 group is more intense compared to HS5 and HS0 groups. Breast meat was evaluated as the best in terms of odour for HS15 group compared to HS0. The colour of thigh meat was better rated in the HSE supplemented groups compared to the controls. In conclusion, dietary supplementation with hempseed expellers appears to affect the colour and odour of broiler chicken's meat which is positive for the consumers. Including hempseed cakes can be recommended as a component of broiler chicken's feed.

This study was conducted to evaluate the effect of feeding milk thistle (Silybum marianum L.) seed cakes at dose 5% and 15% in feed mixture on quality indicators of broiler chickens meat. The used milk thistle seed cakes contained 3.73% of flavonolignans and 129.83 mg.kg -1 of cyanidin-3-glucoside. A 150 cockerels of Ross 308 were divided into three equal groups. The chickens were fattened on conventional deep litter system. The experimental groups received feed mixtures containing 5% of milk thistle seed cakes (MT5), 15% of milk thistle seed cakes (MT15) and third group was controlwithout milk thistle seed cakes (C). The trial lasted 37 days. At the end of trial was observed significant higher average weight of chickens (2,320.31 g) in control group. Compare to that the experimental group MT5 achieved significant lower mean bodyweight 2,166.69 g. From the perspective of fattening was decreased growth of chickens where a higher percentage of milk thistle seed cakes (MT15). The group MT15 was up to 420 g lower slaughter weight compared to the control group. This was probably due to the higher content of fiber in the feed. At the end of experiment 15 birds were selected randomly from each group, weighed and slaughtered. Feathers were removed and chickens were eviscerated. Carcass yield was calculated for each group like as percentage of live weight. The MT5 and MT15 group had significantly higher breast meat tenderness that the control group. Initial pH1 was highest in group with its middle addition of milk thistle seed cakes (MT5). Significant differences were not observed between control and group MT15. Breast meat was rated as the best in parameter flavour in control and MT15 group. The thigh meat was evaluated significantly best for colour parameter in MT15 group. Fibreness was rated as the finest in MT15 group. The addition of milk thistle seed cakes do not worsened sensory characteristic of breast or thigh meat of broilers and reflects optimal sensory quality traits.

The influence of anthocyanins on antioxidant activity and liver function was measured in three different animal species (rats, chickens and fish). Purple Konini wheat with the total anthocyanin content (TAC) of 41.70 mg/kg was fed to the experimental groups of animals (rats n = 32; chickens n = 32; fish n = 20). The same numbers of animals (control groups) were fed common wheat with TAC of 24.95 mg/kg. At the end of the experiment, the animals were killed and blood and liver tissues were taken for biochemical analyses. The liver enzyme activities were measured in blood. Antioxidant activity in the liver tissue was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl test), FR (Free Radicals method), FRAP (Ferric Reducing Antioxidant Power method), and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) test. Significantly higher values (P < 0.05) measured by the DPPH and FR methods were determined in the liver from rats fed Konini wheat. The chickens fed Konini wheat showed significantly different values using DPPH, FR and ABTS methods. No significant differences were found in hepatopancreases of fish. Animals fed Konini wheat showed a tendency to lower liver enzyme activities in general, but a significant difference was found only in gamma-glutamyl transferase activity in the chicken's blood. Our results suggest that feeding a higher content of anthocyanins can improve the antioxidant activity and function of the liver tissue. Rats, chickens, fish, free radicals, radical scavenging activityBesides the contents of nutrients and toxic substances in rations, researchers lately increasingly focus on the biologically active substances with protective and preventive effects against the degenerative diseases and other health problems that may lead to the shortening of life (Ficco et al. 2014;Kaur et al. 2014). In recent years, much attention has been paid to researching a number of substances with antioxidant action, which include vitamins, microelements, and many other compounds naturally occurring in plants and producing positive effects both in human and animal nutrition (Abdel-Aal and Hucl 2003;Englmaierová et al. 2011;Suchý et al. 2014;Sun et al. 2014). Health benefits have been partly attributed to the unique phytochemicals present in the whole grain (Liu 2007). Flavonoids possess antioxidant properties against reactive oxygen species.Antiallergic, anti-inflammatory, and analgesic effects were also demonstrated, including the ability to affect nutrient transfer at the capillary level (Opletal and Skřivanová 2010). In crop species, the edible organs show large variations in colour. In the wheat grain, which is a staple food for humans and grain feed for animals, the colour is mainly due to natural classes of pigment: carotenoids and anthocyanins. Anthocyanins accumulate in the aleurone or pericarp of wheat and provide the blue, purple and red colours of the grain (Ficco et al. 2014). Anthocyanins could be the major compounds distinguishing purple wheat from other coloured wheats for their high...

The experiment was conducted to determine the effect of reduced supplemental zinc levels on broiler growth and carcass yield. A total of 160 male broiler chicks (Ross 308) divided into four groups were allotted to 16 cages with 10 birds per cage in each of group and kept in a temperature-controlled room. During the trial, chicks were ad libitum access to feed and water. The experiment started at 11 days of broiler age and chicks were fattened up to 35 days of age. It consisted of 4 dietary treatments with 4 replications per treatment. A corn-wheat-soybean meal basal diet containing 25.84 mg Zn.kg-1 was formulated and zinc levels of 120, 40 or 20 mg.kg-1 was supplied as zinc oxide to give four dietary treatments. At the end of the feeding trial, 24 birds from each group were randomly selected, slaughtered and carcass evaluation was performed. The results show that different levels of zinc had no significant effect on body weight of broilers or feed consumption ratio. These parameters increased by decreasing zinc levels from 120 to 20 mg Zn.kg-1 similarly as the carcass yield, percentages of breast meat and leg meat, but differences between these groups were not significant. In case of relative liver weight and zinc concentration in liver there were significant difference (p <0.05) between group given supplemented zinc of 40 mg.kg-1 and group without zinc supplementation and 120 mg.kg-1 and 40 mg.kg-1, respectively. No signs of disorders such as loss of appetite, growth depression or abnormalities of the skin was appeared in chicks. It seems that reduced supplemented zinc levels from 120 to 20 mg.kg-1 (total Zn 153.13 mg.kg-1 to 45.28 mg.kg-1 respectively) not influenced growth performance parameters of broilers fed corn-wheat-soybean meal diet.

Aim of the experiment was evaluation of the effect of reduced calcium and magnesium content in the broiler chickens diet on its parameters of fattening, bone strength and calcium and magnesium content in liver. The trial was performed with cockerels of Ross 308 hybrid (n = 160) which were fattened in cage batteries from day 11 th to 36 th day of age. Cockerels were divided into 4 groups (differ in various intake levels of calcium and magnesium) in four replications. The maizewheat-soybean basal diet contained 2.33 g Ca and 1.58 g Mg per kilogram. Calcium was added by CaCO 3 and magnesium by MgSO 4 . Control group (C) received feed mixture with added CaCO 3 in dose of 19.49 g.kg -1 and 0.41 g.kg -1 of MgSO 4 . Three experimental groups contain added CaCO 3 in dose of 11.83 g.kg -1 and 0 g.kg -1 MgSO 4 (group Exp1); CaCO 3 11.83 g.kg -1 and 0.41 g.kg -1 MgSO 4 (group Exp2); CaCO 3 19.49 g.kg -1 and 0 g.kg -1 MgSO 4 (group Exp3), respectively. The feed consumption was daily monitored and the cockerels were weighed twice a week. At the end of the study the experimental animals were weighted and slaughtered by decapitation. The weight of carcasses, liver and proportion of breast and thigh muscle was determined in the selected chickens (n = 24). The atomic absorption spectrometry was used for Ca and Mg evaluation in liver tissues. Bone strength parameter was measured at the femur bone. The statistically significant differences (p >0.05) were not detected between control and experimental groups in the case of studied parameters of fattening, bone strength and calcium and magnesium content in the chicken´s liver. Based on the obtained results it could be concluded the reduction of determined elements in the chicken diet did not deteriorate parameters of yield, elements content in liver tissue as well as the bone strength of broiler chickens.