Temperature changes affect fish behavior, and it is one of the critical factors which influence fish survival. The optimum temperature of a species can be used to detect the presence of fish. The fish heart rate indicates the effect of temperature on fish physiology. This study aims to determine the effect of temperature on the fish heart rate and its recovery time. The study collected the heart rate data by observing 40 eels (Anguila bicolor ) measuring 36±0.30 cm and body weight <200 g at 21, 25, and 31°C. The heart rate was monitored by the electrocardiograph (ECG) technique. The results from video monitoring were calculated using frame-by-frame video analysis. The effect of the temperature on the heart rate and recovery time were processed using statistical analysis with a completely randomized design. The results showed that the eel heart rates at 21, 25, and 31°C were 40±1.61, 70±3.91, and 122±7.36 bpm, respectively. Temperature significantly affected (P<0.05) the eel heart rate with the lower Q10 values were 2.77 and 2.25. The eel heart rate recovery time at low temperature was more prolonged than at high temperature.
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