Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60-80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip enzyme was stable at a pH range 2.6-6.0 and had an optimum pH at 4.0. The enzyme was stable up to 55 °C with an optimum activity at 35 °C.
Ethylene absorption from an ethylene-ethane gaseous mixture in the silver nitrate solution is studied at different temperatures and concentrations. Unlike the previous studies, in which the absorption was studied by batch processes, a semi-continuous process is applied in the present research. The results show that increase in temperature reduces the amount of absorbed ethylene and absorption time. The amount of absorbed ethylene is increased in solutions with higher concentrations of AgNO 3 , whereas the mole ratio of absorbed ethylene per silver nitrate is decreased by increasing the AgNO 3 concentration. Total absorption is modelled as a function of the temperature and concentration of absorbing solution. The estimated values from the model are in good agreement with the experimental data.L'absorption du mélange gazeux de l'éthylène-éthane dans une solution de nitrate d'argent estétudiéeà différentes températures et concentrations. Contrairement auxétudes antérieures lors desquelles l'absorptionétaitétudiée par procédés en lots, un procédé semi-continu a servi pour la recherche actuelle. Les résultats indiquent qu'une hausse de température réduit la quantité d'éthylène absorbée et le temps d'absorption. La quantité d'éthylène absorbée est augmentée dans les solutions ayant des concentrations de AgNO3 plusélevées alors que le ratio de moles d'éthylène absorbés par nitrate d'argent est diminué en augmentant la concentration de AgNO3. L'absorption totale est modélisée en tant que fonction de la température et de la concentration de la solution absorbante. Les valeurs estiméesà partir du modèle appuient les données expérimentales.
N-methyl 2-pyrrolidone (NMP) is applied as a solvent in 1,3-butadiene (BD) extraction plants. The solvent, which is circulated through the unit, is regenerated in a solvent recovery unit. The wastes of solvent recovery unit contains at least 50 wt% NMP which is generally disposed. The recovery of NMP from this waste for reusing in the BD purification plant was studied in the present research by experimental methods such as dissolution, coagulation, and distillation. A purified NMP with the concentration of 99 wt% could be obtained. In addition, the process was scaled up for manufacturing and installing in the BD extraction plant.
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