When Rhizobium japonicum strain 61A68 was added to surface-sterilized soybean ( Glycine max ) seed along with 12 different coating materials, a definite effect of temperature upon survival was observed both with and without coating materials. At a storage temperature of 15°C and 50 ± 5% relative humidity, from 0.9 to 14.1% of the original inoculum survived for 3 weeks. At 22.5°C, from 0.5 to 7.2% of the original inoculum survived. At 30°C, from 0.1 to 1.6% of the original inoculum survived. The data indicated that extremely large numbers of R. japonicum would have to be added to the seed to have numbers adequate for nodulation survive for 3 weeks of storage at ordinary temperatures.
H IGH germinating power of the seed is one among several important factors in obtaining a good stand of flax. The viability of Kansas-grown flaxseed, some of the causes of low germination, and the effect of treating seed on emergence and yield are discussed in this paper. REVIEW OF LITERATURE Landon (I I) 3 stated that good flax seed should have a germination of more than 95%. He stated further that flax seed was more subject to weather damage than wheat or oats, and in some years the weather conditions at the time of ripening may be such that seed of low germination is produced. Dillman and Stoa (5) advised keeping the seed bolls off damp ground after harvest and cautioned that flax intended for seeding purposes should be recleaned before storing, thus removing shriveled and broken seeds, weed seed, and broken stems. Dillman and Toole (6) reported that flax seed of good quality stored under favorable conditions maintained a high degree of viability for IO years or more, but weather-damaged or low-quality seed lost its viability rapidly, beginning with the first year, and in extreme cases was worthless for planting. Seed stored with more than I 1% moisture gave low germination only 6 months after harvest. The variety Linota gave somewhat higher germination than Bison in the tests. Decker and Reitz (3) showed that viability of stored flax declined rapidly moisture and temperature levels were high. Stored with 13% moisture, Linota flax lost its viability completely after 60 days at 86°F but showed only slight decline when stored at 4°0 or 7o°. Lower moisture levels prolonged viability at all temperatures. Dillman (4) found that seeds collected and cured from bolls 9 to 12 days after flowering did not germinate.between blotters in a moist chamber. Those collected at later periods showed germinations of 38% after 15 days, 8o% after 18 days, 9o% after 24 days, and an average of 95% for samples harvested 27 to 36 days after flowering. Robinson (I7) obtained similar results on bulk seed samples from whole plants instead of selected bolls of known-age. Samples taken at weekly intervals, beginning with the full bloom stage, gave germinations of 4.7, 61.3, 82.6, and 89.6%, respectively, indicating the low viability of immature seeds. These samples were cured slowly in the shade while comparable samples cured in the sunlight in a greenhouse germinated o, 7.3, 15.3, and 93.6%, respectively. Fanning the seed sometimes increased the germination lO% or more as a result of removing light weight, underdeveloped seeds. Landon (I 1) explained that low yields of Bison in some of his tests were due low germination of the seed and subsequent poor stands. Davidson and L~ude (2) reported that more Bison seeds were cracked during threshing than was the case with small-seed varieties such as Linota. Bison seed frequently was lower in test weight per bushel than Linota, indicating somewhat poorer development of the seed. Stevens (19) tested I I samples of Bison flax seed showing four types of mechanical injury. His results from soil tests ...
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