Topical autologous platelets have no significant adjuvant effect on healing of chronic venous leg ulcers and increased wound fluid TIMP-1 concentration. Ulcer healing is associated with a decrease in wound fluid IL-8.

A quantitative method of skin healing assessment using true color image processing is presented. The method was developed during a clinical trial using healthy volunteers, the goal of which was to study a drug for accelerating healing. Photographic images of the skin were sequentially acquired between day 1 and day 12 after pure painless epidermal wounds. The images were digitized in controlled conditions using a color video camera connected to a computer system. A color threshold based segmentation was developed to provide an operator-independent delineation of the wound. Two healing indexes were built measuring, the wound area and the wound color. The method was implemented in a software system allowing a fully automated determination of the healing indexes. The method provides a new quantitative global assessment of healing kinetics. It is noninvasive and well suited for multicentric clinical trials.

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To define a healing function based on parameters measured on digitized images of wounds, and to use it to compare the rate of healing of two skin graft donor sites, one treated with petrolatum gauze (Pg) and the other with a topical preparation containing alginates (A). Digital photographs of donor sites (depth 0.6 mm) taken every two days between day 6 and day 12 were analyzed blind using the same algorithm, following changes in color and homogeneity. Analysis of variance was used to identify those parameters that changed during healing. The healing function was constructed using measurements made in six patients (group 1) randomly chosen from ten requiring skin grafts, and was applied and validated using data from the remaining four patients (group 2). The results given by this healing function were compared with those provided by principal component analysis. The most significant healing parameters were those measuring wound homogeneity, and our healing function reflects how these change with time. The time-dependent curves of the function calculated for groups 1 and 2 matched well enough to be considered as being derived from the same set of measurements. The results given by this healing function explained, by analogy, the meaning of the first principal component of principal component analysis. From day 6 to day 12, the healing function followed the same time-course for the Pg and A treatments, but healing was achieved significantly earlier (4 days, p < 0.03) with A. This suggests that the effect of A on wound healing is achieved in the first six days, before the visual changes from epidermalization are analyzable.

Because of conflicting reports on the photoxicity of rhodamine 123 (Rh 123), we have undertaken a study of Rh 123 photosensitization in several in vitro systems. First, Rh 123 is not a photodynamic agent and does not react with singlet oxygen. Second, when bound to cytochrome c (Cyt c), Rh 123 photosensitizes ferro Cyt c but not ferri Cyt c degradation by an oxygen-independent process. When delivered to skin fibroblasts where it specifically stains mitochondria, Rh 123 photosensitizes membrane damage. These results are consistent with the hypothesis that Rh 123 is a phototoxic stain. The lack of photosensitivity of Rh 123-stained mitochondria in some cell lines might therefore be due to specific structural features.

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Dihydrofolate reductase (DHFR) is an intracellular enzyme which catalyzes the NADPH-dependent reduction of dihydrofolate. Inhibition of the enzyme,1} e.g., by chemotherapeutic drugs like methotrexate (MTX), de-

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