Background: Background:Treatment of acute myeloid leukemia (AML) remains difficult to this day, with high rates of relapse that lead to poor long-term prognosis and low cure rates and are usually driven by residual leukemic cells that can be identified as measurable residual disease (MRD). Multiparametric Flow Cytometry (MFC) has been a fast and effective tool for detection of MRD, but disease heterogeneity makes MRD assessment a clinical challenge, especially in cases with rare phenotype, needing clinical and technical expertise to be accurately performed.
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