To mention the role of ticks in transmission of Klebsiella spp. and Staphylococcus aureus, sixty seven engorged adult females of the type which called hard ticks (Rhipicephalus spp.) were collected from cattle and sheep randomly from diverse regions of Baghdad city through the period November 2015 till March 2016. Blood swabs from ticks were subjected to the culture media to determine the percentages of Klebsiella spp. and Staphylococcus aureus. Results showed that out of 67 samples, 13 (14.40%) samples were positive for Klebsiella spp., 16 (23.88%) samples were positive for Staphylococcus aureus and 8 samples were positive for both bacteria. This study revealed that hard ticks (Rhipicephalus spp.) is a good source for bacterial infection by Klebsiella spp. and Staphylococcus aureus .

     This study was designed to isolate of Enterohaemoragic E.coli O157:H7 from feces and urine in cattle. 175 samples (80 fecal and 95 urine) were collected to isolate EHEC O157:H7 during the period from March to June 2014 from Al- Hindia slaughter house from local breeding cattle, aged between 1-2 years and over 2 years, and the samples were subjected to culture on specific media Cefixime Tellurit- Sorbitol MacConkey agar at 37 ˚C for 18-24 hrs. then the isolates were identified by biochemical tests (Indol test, Potassium cyanid test) and seriologically by Latex Kit test. The results showed that 73(91.25%) out of 80 fecal samples and 39(41%) out of 95 urine samples were E.coli O157:H7 positive, the total of positive isolates were 64%. That reveals the important role of feces and urine samples to contaminate the environment and disseminate of infection.

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