Organoid models have gained importance in recent years in determining the toxic effects of drugs in cancer studies. Organoid designs with the same standardized size and cellular structures are desired for drug tests. The field of microfluidics offers numerous advantages to enable well-controlled and contamination-free biomedical research. In this study, simple and low-cost microfluidic devices were designed and fabricated to develop an organoid model for drug testing for renal cancers. Caki human renal cancer cells and mesenchymal stem cells isolated from human umbilical cord were placed into alginate hydrogels. The microfluidic system was implemented to form size-controllable organoids within alginate hydrogels. Alginate capsules of uniform sizes formed in the microfluidic system were kept in cell culture for 21 days, and their organoid development was studied with calcein staining. Cisplatin was used as a standard chemotherapeutic, and organoid sphere structures were examined as a function of time with an MTT assay. HIF-1α, CXCR4 and CXCL-12 chemokine protein, and CXCR4 and CXCL-12 gene levels were tested in organoids and cisplatin responses. In conclusion, it was found that the standard renal cancer organoids made on a lab-on-a-chip system can be used to measure drug effects and tumor microenvironment responses.
The umbilical cord (UC) is a rich source of mesenchymal stem cell (MSC) isolation. Since the MSCs isolated from here have high self-renewal capacity and differentiation potential, production through biofabrication is essential for clinical treatments. For the cells to be stored for a long time and presented ready for use, encapsulation is required. In this study, UC-MSC cells were encapsulated with alginate using three different methods: alginate drop, alginate coating, and alginate sphere. Methods: The cell viability, live/dead cell ratio, and colony formation capacities of the encapsulated cells were examined for 14 days. Results: In the study, it was found that the most effective method was the alginate sphere form and that the structure of the cells should be preserved by injecting them into biomaterials in encapsulation. Colony formation potential was found to be high in biomaterials with alginate spheres. Conclusion: As a result, the preservation of UC-MSC cells with alginate sphere encapsulation via biofabrication and their clinical use availability may be beneficial for treating of many diseases.
Bu çalışmada Aydın Adnan Menderes Üniversitesi’nin tüm kampüslerinde afet ve acil durumlara müdahale kapsamında destek personelin hazır oluşluğunun arttırılmasına yönelik online teorik eğitim ile yüzyüze uygulamalı eğitim verilmesi amaçlanmıştır. Ön test-son test deseninin uygulandığı müdahale tipinde bir çalışmadır. Araştırmanın örneklemini Mayıs 2020 – Ağustos 2021 arasında üniversitenin her biriminde (Rektörlük, enstitü, fakülte, yüksekokul, uygulama ve araştırma merkezi, hastane vb.) destek elemanı olarak görevlendirilen toplam 679 personel oluşturdu. Araştırma verileri Birey Tanıtım Formu ve Destek Elemanlarının Afet ve Acil Durumlara Hazır Oluşluğuna Yönelik (Tahliye ve Arama Kurtarma, Yangın) Bilgi Testleri ile toplanmıştır. Verilerin değerlendirilmesinde bağımlı gruplarda t test, Student t testi kullanılmıştır. Katılımcıların Tahliye Arama ve Kurtarma Bilgi Testi Ön-test skor ortalaması 11,5±2,2 iken, Son-test skor ortalaması 13,4±2,7 olarak saptanmıştır. Katılımcıların Tahliye Arama ve Kurtarma Bilgi Testi Ön-test ve Son-test skor ortalamaları arasındaki fark anlamlı olarak bulunmuştur (z = -16,537, p < 0,001). Personellerin Yangın Bilgi Testi Ön-test skor ortalaması 11,5 ± 2,2 iken, Son-test skor ortalaması 13,3 ± 2,7 olarak saptanmıştır. Katılımcıların Tahliye Arama ve Kurtarma Bilgi Testi Ön-test ve Son-test skor ortalamaları arasındaki fark anlamlı olarak belirlenmiştir (z = -15,017, p < 0,001). Verilen eğitimin katılımcıların bilgi düzeylerinde artış sağladığı sonucuna ulaşılmıştır. Üniversitelerin bünyelerinde çalışan akademik ve idari personelden oluşan destek elemanlarına yönelik olarak verilen bu eğitimlerin ülkemiz genelinde yaygınlaştırılması önerilmektedir.
Aim: Cancer stem cells are cell populations that are essential in drug resistance and cancer metastasis. Some liver cancer cells exhibit the characteristics of cancer stem cells, and it is crucial to study the activities and interactions of drugs in these cells. Huh7 is a human liver cancer cell with stem cell biomarkers and is used with induced pluripotent stem cells to form various cancer organoids through encapsulation methods. Due to their ease of use without animal testing, bio-fabrication studies of cell-encapsulated models have gained importance in the pharmaceutical industry in recent years. This study aimed to biofabricate Huh7 human liver cancer stem cells by alginate encapsulation and test gemcitabine’s efficacy. Methods: Huh7 cells were encapsulated with alginate (0.8% w/v) and fibronectin, and their viability was evaluated with 3.2 µM gemcitabine on days 1, 3, 6, 9, and 12. Furthermore, gene expressions of stem cell markers CD90 and AFP were evaluated in encapsulated Huh7 cells by qPCR. In addition, IL-6 secretion in the cell medium was measured by ELISA for the tumor microenvironment. Results: Encapsulation of Huh7 cells was found to maintain their viability and stem cell properties for up to 12 days. In addition, alginate-encapsulated Huh7 cells were bio-fabricated to demonstrate long-term gemcitabine response. While the effect of the gemcitabine was evaluated in alginate-encapsulated Huh7 cells, CD90 and AFP mRNA levels were significantly reduced in the cells and IL-6 secretion was decreased in the tumor microenvironment. Conclusion: This study demonstrated that bio-fabrication of alginate-encapsulated Huh7 cells is a novel approach for long-term drug testing in liver cancer models. Bio-fabricated alginate-encapsulated cancer stem cells may be a cheaper and faster method for the testing of many drugs.
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