) doses of MPD on mixed isolates of P. infestans produced no isolates resistant to the compound. The results of this study indicate that the probability of a buildup of resistant sub-populations of P. infestans to mandipropamid in the field is low.

An improved, soil-free laboratory method was developed to induce late blight infection in detached tomato leaves or tomato seedlings with in vivo-produced oospores of Phytophthora infestans. Oospores were produced in detached tomato leaves infected with an inoculum mixture of A1 and A2 isolates. The infected leaves were homogenized in distilled water. The homogenates were exposed to two cycles of drying and wetting to kill sporangia, zoospores, and mycelia and, thereafter, mixed with perlite and water. Tomato leaves or seed were floated on the perlite mixture in petri dishes and incubated for up to 30 days. Results from 13 experiments showed that, in dishes containing oospores, 96 of 820 leaflets (11.7%) developed late blight lesions within 6 to 30 days; whereas, in 3 experiments with seedlings, 12 of 1,400 plants (0.9%) were blighted within 17 to 26 days. No late blight developed in leaflets or seedlings maintained in dishes containing homogenates of the control leaves infected with either A1 or A2 inoculum. In all, 234 single-sporangium F1 isolates recovered from 24 leaf lesions produced by four crosses and 27 isolates recovered from 12 seedlings infected with two crosses were examined for sensitivity to metalaxyl and mating type. Results showed that, although the parent isolates were either S (sensitive) or R (resistant) to metalaxyl, most F1 progeny isolates exhibited various levels of intermediate resistance to metalaxyl. Most isolates belonged to either the A1 or the A2 mating type, depending on the cross and lesion. However, some isolates belonged to the unusual mating type A1A2 or were sterile. Current experiments are intended to elucidate the virulence and aggressiveness of these F1 progenies to tomato. This improved method may facilitate our understanding of the role of in vivo-produced oospores in the epidemiology of late blight in tomato.

Tomato fruits at the mature green stage coinoculated with A1 + A2 sporangia of Phytophthora infestans, the late blight causal fungus, showed abundant oospores in the vascular tissues, pericarp, columella, and placenta. Oospores were also formed on the surface of fruits kept in moisture-saturated atmosphere. Occasionally, oospores were enclosed between the epidermal hairs of the seed coat. In a few seeds, oospores were detected inside the embryo. The data suggest that blighted tomato fruits may carry a large number of oospores, thus making them a threatening source of blight inoculum. Such fruits may also release airborne oosporic inoculum that may introduce recombinant genotypes within a growing season. Although Phytophthora infestans is seedborne in tomato, to our knowledge, this is the first report on the occurrence of oospores in tomato seeds. Whether such tomato seeds produce blighted seedlings remains to be shown.

No abstract

The ability of Phytophthora infestans, the causal agent of potato and tomato late blight, to produce oospores in potato tuber tissue was studied in the field and under laboratory conditions. In 1998 and 2000 field experiments, the canopy of potato cvs. Alpha and Mondial, respectively, were coinoculated with A1 + A2 sporangia of the fungus, and the infected tubers collected at harvest were examined for the presence of oospores. In 1998, only 2 of 90 infected tubers had oospores, whereas none of the 90 tubers examined in 2000 had any oospores. In the latter experiment, infected tubers kept in storage up to 12 weeks after harvest had no oospores. Artificial co-inoculations of whole tubers with A1 + A2 sporangia resulted only rarely in the formation of oospores inside the tubers. Co-inoculations of potato tuber discs taken from dormant tubers 0 to 16 weeks after harvest failed to support any oospore production, whereas discs taken from sprouting tubers of >/=18 weeks after harvest allowed oospores to form. Tuber discs showed enhanced oospore formation when treated before inoculation with either sugars, amino acids, casein hydrolysate, beta-sitosterol, or chloroethylphosphonic acid. In contrast, reducing airflow into the petri dishes where potato tuber discs were incubated reduced the number of oospores produced. The number of oospores produced in tuber tissue was lower compared with that in leaf tissue regardless of the origin of isolates used. The data show that the ability of Phytophthora infestans to produce oospores in potato tuber tissue is very limited and increases with tuber aging.