Antimicrobial peptides are a promising alternative to traditional antibiotics, but their utility is limited by high production costs and poor bioavailability profiles. Bacterial production and delivery of antimicrobial peptides (AMPs) directly at the site of infection may offer a path for effective therapeutic application. In this study, we have developed a vector that can be used for the production and secretion of seven antimicrobial peptides from both Escherichia coli MC1061 F’ and probiotic E. coli Nissle 1917. The vector pMPES (Modular Peptide Expression System) employs the Microcin V (MccV) secretion system and a powerful synthetic promoter to drive AMP production. Herein, we demonstrate the capacity of pMPES to produce inhibitory levels of MccV, Microcin L (MccL), Microcin N (McnN), Enterocin A (EntA), Enterocin P (EntP), Hiracin JM79 (HirJM79) and Enterocin B (EntB). To our knowledge, this is the first demonstration of such a broadly-applicable secretion system for AMP production. This type of modular expression system could expedite the development of sorely needed antimicrobial technologies.

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Context. The combination of large segmented space telescopes, coronagraphy, and wavefront control methods is a promising solution for producing a dark hole (DH) region in the coronagraphic image of an observed star in order to study planetary companions. The thermal and mechanical evolution of such a high-contrast instrumental setup leads to wavefront drifts that degrade the DH contrast during the observing time, thus limiting the ability to retrieve planetary signals. Aims. Lyot-style coronagraphs are starlight-suppression systems that remove the central part of the image for an unresolved observed star, that is, the point spread function, with an opaque focal plane mask (FPM). When implemented with a flat mirror containing an etched pinhole, the mask rejects part of the starlight through the pinhole which can be used to retrieve information about low-order aberrations. Methods. We propose an active control scheme using a Zernike wavefront sensor (ZWFS) to analyze the light rejected by the FPM, control low-order aberrations, and stabilize the DH contrast. We first present the concept formalism and then describe how we characterized the sensor behavior in simulations and in the laboratory. We performed experimental tests to validate a wavefront control loop using a ZWFS on the HiCAT testbed. Results. By controlling the first 11 Zernike modes, we show a decrease in the standard deviation of the wavefront error by a factor of up to 9 between open- and closed-loop operations using the ZWFS. In the presence of wavefront perturbations, we show the ability of this control loop to stabilize a DH contrast around 7 × 10−8 with a standard deviation of 7 × 10−9. Conclusions. Active control with a ZWFS proves to be a promising solution in Lyot coronagraphs with an FPM-filtered beam for controlling and stabilizing low-order wavefront aberrations and DH contrast for exoplanet imaging with future space missions.

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