Using a free-air CO2 enrichment (FACE) experiment, poplar trees (Populus x euramericana clone I214) were exposed to either ambient or elevated [CO2] from planting, for a 5-year period during canopy development, closure, coppice and re-growth. In each year, measurements were taken of stomatal density (SD, number mm(-2)) and stomatal index (SI, the proportion of epidermal cells forming stomata). In year 5, measurements were also taken of leaf stomatal conductance (gs, micromol m(-2) s(-1)), photosynthetic CO2 fixation (A, mmol m(-2) s(-1)), instantaneous water-use efficiency (A/E) and the ratio of intercellular to atmospheric CO2 (Ci:Ca). Elevated [CO2] caused reductions in SI in the first year, and in SD in the first 2 years, when the canopy was largely open. In following years, when the canopy had closed, elevated [CO2] had no detectable effects on stomatal numbers or index. In contrast, even after 5 years of exposure to elevated [CO2], gs was reduced, A/E was stimulated, and Ci:Ca was reduced relative to ambient [CO2]. These outcomes from the long-term realistic field conditions of this forest FACE experiment suggest that stomatal numbers (SD and SI) had no role in determining the improved instantaneous leaf-level efficiency of water use under elevated [CO2]. We propose that altered cuticular development during canopy closure may partially explain the changing response of stomata to elevated [CO2], although the mechanism for this remains obscure.
Spatial and daily variation in photosynthetic water-use efficiency was examined in leaves of Betula pendula Roth with respect to distribution of hydraulic conductance within the crown, morphological properties of stomata, and water availability. Intrinsic water-use efficiency (A n /g s ) was determined from gas-exchange measurements performed both in situ in a natural forest stand and on detached shoots under laboratory conditions. In intact foliage, sun leaves demonstrated significantly higher (P < 0.001) A n /g s than shade leaves, as photosynthesis in the lower canopy was chronically limited by low light availability. However, this difference reversed in the mid-day period under sufficient irradiance (I > 800 lmol m À2 s À1 ): A n /g s averaged 28.8 and 24.0 lmol mol À1 (P < 0.01) for shade and sun leaves, respectively. This last finding coincided with the data obtained in laboratory conditions: under equivalent leaf water supply and light, A n /g s in shade foliage was greater (P < 0.001) than in sun foliage across a wide range of irradiance. Thus, shade foliage of B. pendula is characterized by inherently higher A n /g s than sun foliage, associated with more conservative stomatal behavior, and lower soil-to-leaf (K T ) and leaf hydraulic conductances. Under unlimited light conditions, a within-crown trade-off between A n /g s and K T becomes apparent. Differences in stomatal conductance between the detached shoots from sunlit and shaded canopy layers were largely attributable to the variation in stomatal morphology; significant relationships were established with characteristics combining stomatal size and density (relative stomatal surface, stomatal pore area index). Stomatal morphology is very likely involved in long-term adjustment of photosynthetic WUE.
Stomatal density and size were measured along the light gradient of a Betula pendula Roth. canopy in relation to microclimatic conditions. The theoretical stomatal conductance was calculated using stomatal density and dimensions to predict to what degree stomatal conductance is related to anatomical properties and relative stomatal opening. Stomatal density was higher and leaf area smaller in the upper canopy, whereas epidermal cell density did not change significantly along the canopy light gradient, indicating that stomatal initiation is responsible for differences in stomatal density. Stomatal dimensions – the length of guard cell on the dorsal side and the guard cell width – decreased with declining light availability. Maximum measured stomatal conductance and modelled stomatal conductance were higher at the top of the crown. The stomata operate closer to their maximum openness and stomatal morphology is a more important determinant of stomatal conductance in the top leaves than in leaves of lower canopy. As stomata usually limit photosynthesis more in upper than in lower canopy, it was concluded that stomatal morphology can principally be important for photosynthesis limitation in upper canopy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.