The relationships between crayfish species abundance patterns during summer baseflow conditions and environmental variables at the watershed and reach scales were assessed across 30 study reaches in Kentucky's upper Green River basin, U.S.A. Mixed gravel-cobble and large boulder substrates were sampled from each reach. Six species in total were obtained. Orconectes rusticus and Orconectesputnami were obtained from 29 reaches each and combined comprised 92.6% of the total individuals collected. The most common Cambarus (C. graysoni) constituted 4.8 % of the total individuals obtained. Although 0. putnami and C. graysoni had higher mean density values on large boulders, an independent measure t-test revealed non-significant differences. A similar result occurred for 0. rusticus, with only slightly higher densities on gravel-cobble substrates. A canonical correspondence analysis (CCA) in the forward selection procedure reduced the number of environmental variables from 27 to five for the gravel-cobble data only. The second CCA revealed relationships between crayfish species abundance with percentage of urban land-use and stream-size related variables (e.g., temperature). Pearson correlations showed that density of only 0. rusticus was related to percentage of urban land-use and temperature.
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