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This study aimed to determine the minimum anesthetic concentration (MAC) and dose-related cardiovascular effects of isoflurane during controlled ventilation in cinereous vultures (Aegypius monachus). The MAC was determined for 10 cinereous vultures as the midpoint between the end-tidal isoflurane concentration that allows gross purposeful movement and that which prevents the movement in response to clamping a pedal digit. Immediately after the MAC was determined, the cardiovascular effects of isoflurane at 1.0, 1.5, and 2.0 times the MAC were investigated in seven of the 10 birds. The MAC of isoflurane for 10 cinereous vultures during controlled ventilation was 1.06 +/- 0.07% (mean +/- SD). When the isoflurane concentration was increased to 1.5 and 2.0 times the MAC, there was significant dose-dependent decrease in the arterial blood pressure. However, the heart rate did not change over a range of 1.0 to 2.0 times the MAC.

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ABSTRACT. The aim of this study was to determine whether socialized Jindo puppies would show different behavioral reactivity from nonsocialized puppies. Puppies (n=12), 7 weeks of age, were divided into socialized and non-socialized groups. The socialized group from the 7th until 13th week after birth was provided a socialization program, and the non-socialized group was reared in a semi-isolated environment without being exposed to the program. At 13 weeks after birth, both groups were adopted by new families and raised as a family pet until adulthood. Both groups were tested in 5 behavioral tests at 7, 9, 11, 13 and 60 weeks of age, and their behavioral responses to the tests were recorded using video cameras. The contact, fearful and playful behaviors toward each behavioral test were scored on a scale of 1 to 5 points. Using all of the score data, a principal component analysis (PCA) extracted three primary factors: 'social reactivity towards humans and a dog', 'playful reactivity towards novel stimuli and a dog' and 'fearful reactivity towards social stimuli'. The three extracted factors were compared between the socialized and non-socialized groups in each test session (weeks). Based on the results, the socialized Jindo puppies in the test session at the 9th week after birth, in contrast to the non-socialized puppies, exhibited a higher intensity of playful reactivity towards novel stimuli and a dog. However, there were no effects of the socialization program on the Jindo puppies in terms of social reactivity towards humans and a dog as well as fearful reactivity towards social stimuli.