histology and molecular fungal diagnosis were obtained. The DNA-chip-based technology detected Trichophyton benhamiae, and systemic antifungal administration of fluconazole led to rapid improvement within 11 days. The diagnosis of deep trichophytia was later confirmed by histopathological examination.In all cases, diagnosis of tinea was made using multiplex PCR and histopathological findings (in two cases), whilst microscopic detection was inconclusive, and results from culture were still pending when therapeutic decisions had to be taken. The molecular dematomycosis array is a novel and practical method to detect fungal infections within 1-2 days. It is characterized by a high sensitivity (82%) and specificity (91%). By contrast, the sensitivity of direct microscopy and culture are significantly lower (Bieber et al., submitted). Furthermore, it is a non-invasive method, allows species identification and swabs can be used to obtain material for analysis.Overall, this case series underscores the relevance of molecular diagnosis of fungal skin infections, which in the near future should improve the management tinea.
AcknowledgementThe patients in this manuscript have given written informed consent to the publication of their case details.
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