Antimicrobial peptides of higher organisms have been studied for the past 25 years, and their importance as components of innate immunity is now well established. The essential simplicity of their chemical structure, along with the lower likelihood of developed resistance compared to conventional antibiotics, has made them attractive candidates for development as therapeutics. The objective of this review article is to describe the current relevance, main mechanisms presented, and the uses of antimicrobial peptides as new therapies in the clinical area. The information used was mainly compiled from scientific articles based on a systematic review of scientific papers with data on human antimicrobial peptides (AMPs) and their different applications, searching without date limits and only documents in English and Spanish. Gray literature was accessed through manual search, and no restrictions were made involving study design for a retrospective study. Although these products have not yet been commercialized, they have advantages over the currently available treatments since they are not expected to cause bacterial resistance due to their three-dimensional structure, amphipathic tendency, and cationic character; however, the technique of peptide production is still new and is in the early stages of innovation of new molecules.
Background To test the antimicrobial potential of clove essential oil that has been less investigated on antimicrobial-resistant organisms (extended-spectrum β-lactamase-ESBL-producing Escherichia coli), we collected 135 ESBL-producing Escherichia coli strains given that E. coli is the major organism increasingly isolated as a cause of complicated urinary and gastrointestinal tract infections, which remains an important cause of therapy failure with antibiotics for the medical sector. Then, in this study, we evaluated the relationship between the antibacterial potential activity of Syzygium aromaticum essential oil (EOSA) and the expression of antibiotic-resistant genes (SHV-2, TEM-20) in plasmidic DNA on ESBL-producing E. coli using RT-PCR technique. Results EOSA was obtained by hydrodistillation. Using Kirby-Baüer method, we found that EOSA presented a smaller media (mean = 15.59 mm) in comparison with chloramphenicol (mean = 17.73 mm). Thus, there were significant differences (p < 0.0001). Furthermore, EOSA had an antibacterial activity, particularly on ECB132 (MIC: 10.0 mg/mL and MBC: 80.0 mg/mL), and a bacteriostatic effect by bactericidal kinetic. We found that the expression of antibiotic-resistant gene blaTEM-20 was 23.52% (4/17 strains) and no expression of blaSHV-2. EOSA presented such as majority compounds (eugenol, caryophyllene) using the GC–MS technique. Conclusions Plant essential oils and their active ingredients have potentially high bioactivity against a different target (membranes, cytoplasm, genetic material). In this research, EOSA might become an important adjuvant against urinary and gastrointestinal diseases caused by ESBL-producing E. coli.
Studies on bioactivities of numerous essential oils (EOs) and herbal extracts compounds against diseases are crucial. Microencapsulation methods development might be an alternative to obtain bioactive compounds for cosmetics and pharmaceutical uses. In this study, we carried out a literature review of 219 503 data articles using ScienceDirect, Redalyc, Web of Science, Scopus, SciELO, and Google Scholar databases in English and Spanish, after with inclusion (original articles, book chapters, and theoretical references) and exclusion criteria (frameworks description), we found 1854 restricting the publication years between 2004 and August 2020 and 35 relevant articles with our scope research. References found contained a collection of methods that could be utilized to create microcapsules, including coacervation, extrusion, polymerization, and spray drying. This article analyzed the most recent and advanced microencapsulation techniques and their applications in the food, cosmetic, and pharmaceutical industries. Herbal extracts and EOs have many applications, depending on the wall materials and microencapsulation methods that could help know about selective release and efficacy to ensure optimal dosing and other advantages; thus, improving the profitability of these product manufacturers.
Se analizaron datos de E. coli productora de BLEEs aisladas de pacientes de diferentes áreas hospitalarias por medio de las interacciones que existen entre los antibiogramas y las áreas a través de análisis de varianza (ANOVA) de dos factores y la prueba de Bartlett para homocedasticidad. Se encontró una incidencia del 45% de E. coli y de 135 cepas muestreadas, el 4.58% fue E. coli BLEE. Además, usando una prueba de Bartlett, se determinó que existe una diferencia significativa entre las áreas y en sus pruebas fenotípicas, por lo que se deben implementar medidas de capacitación, control y prevención en el sector salud para evitar en mayor medida la propagación de infecciones entre áreas de salud pública.
Objectives: The alginate microspheres were prepared with methanol extract of rue and essential oil of clove with metronidazole evaluating their biological and physicochemical activities. Materials and methods: Microspheres were evaluated with their sphericity, encapsulation efficiency, degradation, and release kinetics with mathematical models.
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