Background As the average life expectancy increases, skin aging and wrinkles due to photoaging have gained attention. Collagen is closely involved in the process of skin aging.Among the potential methods of drug delivery to the skin, oral disintegrating films show promise for their ability to bypass the loss of active components that is typical of drug absorption via oral administration. This study was conducted to investigate the effect of an oral disintegrating collagen film on skin aging. Methods We performed a prospective, single-arm study in a cohort of 22 women to assess the anti-aging effect of a novel oral disintegrating film containing collagen applied daily over a 12-week period. We measured the clinical indicators of skin integrity and performed immunofluorescence and high-performance liquid chromatography analyses of an ex vivo oral mucosa model to compare the absorption rates of collagen films and conventional oral tablets via the mucosa. ResultsWe found that the oral disintegrating collagen film reduced skin wrinkle depth and number and significantly increased skin elasticity and density. Conclusions The novel mode of delivery of collagen via oral disintegrating films has a clinically potential anti-aging efficacy and is safe and convenient for daily use.
One of the most frequent comorbidities that develop in chronically ill or immobilized patients is pressure ulcers, also known as bed sores. Despite ischemia-reperfusion (I/R)-induced skin lesion having been identified as a primary cause of pressure ulcers, wound management efforts have so far failed to significantly improve outcomes. Baicalin, or 5,6,7-trihydroxyflavone, is a type of flavonoid which has been shown to possess a variety of biological characteristics, including antioxidative and anti-inflammatory effects and protection of I/R injury. In vitro wound scratch assay was first used to assess the function of baicalin in wound healing. We established a mouse model of advanced stage pressure ulcers with repeated cycles of I/R pressure load. In this model, topically applied baicalin (100 mg/mL) induced a significant increase in the wound healing process measured by wound area. Histological examination of the pressure ulcer mouse model showed faster granulation tissue formation and re-epithelization in the baicalin-treated group. Next, baicalin downregulated pro-inflammatory cytokines (IL-6 and IL-1β), while upregulating the anti-inflammatory IL-10. Additionally, baicalin induced an increase in several growth factors (VEGF, FGF-2, PDGF-β, and CTGF), promoting the wound healing process. Our results suggest that baicalin could serve as a promising agent for the treatment of pressures ulcers.
Indirubin is an active constituent of traditional Chinese recipe used for the treatment of chronic myelocytic leukemia. In this study, inhibitory activity of indirubin and its derivatives toward Fms-like tyrosine kinase 3 (FLT3) was examined. Indirubin-3′-oxime(IO) and 6-bromoindirubin-3′-oxime(BIO) showed potent inhibitory activity against FLT3 with 50% inhibitory concentration (IC50) of 79 nM and 254 nM, respectively. Meanwhile, indirubin and 6-bromoindirubin exhibited negligible effect on FLT3 inhibitory activity up to 10 µM. We also tested the cytotoxicity of those compounds against acute myeloid leukemia cell lines; MV4;11 cells harboring constitutively activated form of FLT3 and RS4;11 cells with wild type FLT3. IO and BIO potently inhibited the growth of MV4;11 cells with IC50 of 30 nM and 61 nM, respectively. On the other hand, RS4;11 cells were far less sensitive to those compounds with IC50 values of 3600 nM and 820 nM, respectively. In the cell cycle assay, IO arrested the cell cycle of MV4;11 cells at G1 phase, and increased dead cell population at sub-G1 phase about 25 and 50% at 0.1 and 1.0 µM after 48 hours, respectively. These results strongly suggest that the derivatives of IO have potentials to be developed for novel anti-leukemic agents. Citation Information: Mol Cancer Ther 2009;8(12 Suppl):C200.
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