We propose a nanobiosensor to evaluate a lung cancer-specific biomarker. The nanobiosensor is based on an anodic aluminum oxide (AAO) chip and functions on the principles of localized surface plasmon resonance (LSPR) and interferometry. The poredepth of the fabricated nanoporous AAO chip was 1 m and was obtained using a two-step electrochemical anodization process. The sensor chip is sensitive to the refractive index (RI) changes of the surrounding medium and also provides simple and labelfree detection when specific antibodies are immobilized on the gold-deposited surface of the AAO chip. In order to confirm the effectiveness of the sensor, the antibodies were immobilized on the surface of the AAO chip, and the lung cancer-specific biomarker was applied atop of the immobilized-antibody layer using the self-assembled monolayer method. The nanoporous AAO chip was used as a sensor system to detect serum amyloid A1, which is a lung cancer-specific biomarker. The specific reaction of the antigenantibody contributes to the change in the RI. This in turn causes a shift in the resonance spectrum in the refractive interference pattern. The limit of detection (LOD) was found to be 100 ag/mL and the biosensor had high sensitivity over a wide concentration range.
In this study, we have developed a mobile-based visible/NIR(Near InfraRed) imaging equipment for the animal testing. This equipment can provide visible, NIR and merged image through the viewer program. Especially, merged image help researcher to understand visual messages at animal in-vivo test. Also, it is available to send real-time images through the smart phone. Researcher can communicate with another researcher who is a long distance away. Also, the equipment was made with portable small size to enable it to commercialize.
In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated 2 µm of pore-depth by twostep anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.Keywords : Biosensor, Lung cancer-specific biomarker, AAO, Nano SPR, Interference Received : Feb. 1, 2013, Accepted : Mar. 21, 2013 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License(http://creativecommons.org/licenses/bync/3.0)which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
We have developed an optical gas sensor for detection of volatile organic compounds (VOCs) gases. The side-polished optical fiber coupled with the polymer planar waveguide (PWG) which has high sensitive to change refractive index. The PWG was fabricated by coating the solvatochromic dye with a polyvinyl pyrrolidone (PVP). The fabricated gas sensor system was tested with five-types of VOCs gases. The different polarities of the VOC gases cause changes in the effective refractive index of the sensing membrane owing to evanescent field coupling. According to the results, the proposed gas sensor showed high sensitivity.I.
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