ResumoO objetivo deste trabalho foi avaliar o efeito do óleo essencial de Eucalyptus globulus (OEEG) sobre a eclosão e desenvolvimento de larvas de Haemonchus contortus. A determinação da composição química do OEEG foi feita por meio de cromatografia gasosa acoplada à espectrometria de massa. No teste de eclosão de ovos (TEO), utilizaram-se as concentrações 21,75; 17,4; 8,7; 5,43 . The concentration of EGEO that inhibits 50% of the eggs and larvae was 8.3 and 6.92 mg.mL -1 , respectively. The oil chemical analysis identified as main component the monoterpen 1,8-cineol. EGEO presented ovicidal and larvicidal activities in vitro, revealing a good potential for use in the control of sheep and goat gastrointestinal nematodes.Keywords: Haemonchus contortus, phytoterapic, anthelmintic.
IntroduçãoAs endoparasitoses gastrintestinais constituem-se no principal entrave da ovinocultura, em todo o mundo, especialmente, nas regiões tropicais, onde os prejuízos econômicos são mais acentuados (VIEIRA, 2008). Dentre os parasitos gastrintestinais, destaca-se o nematóide hematófago Haemonchus contortus, por causa da sua alta prevalência e patogenicidade (AROSEMENA et al., 1999). O comprometimento da produção ocorre em decorrência da perda de apetite, diarréia, anemia e em casos severos, morte do animal (ATHANASIADOU; KYRIAZAKIS, 2004).Para tentar controlar e reduzir as perdas causadas pelas infecções por helmintos, são utilizados anti-helmínticos sintéticos
In June 2019, a horse with neurological disorder was diagnosed with West Nile virus (WNV) in Boa Viagem, a municipality in the state of Ceará, northeast Brazil. A multi-institutional task force coordinated by the Brazilian Ministry of Health was deployed to the area for case investigation. A total of 513 biological samples from 78 humans, 157 domestic animals and 278 free-ranging wild birds, as well as 853 adult mosquitoes of 22 species were tested for WNV by highly specific serological and/or molecular tests. No active circulation of WNV was detected in vertebrates or mosquitoes by molecular methods. Previous exposure to WNV was confirmed by seroconversion in domestic birds and by the detection of specific neutralizing antibodies in 44% (11/25) of equids, 20.9% (14/67) of domestic birds, 4.7% (13/278) of free-ranging wild birds, 2.6% (2/78) of humans, and 1.5% (1/65) of small ruminants. Results indicate that not only equines but also humans and different species of domestic animals and wild birds were locally exposed to WNV. The detection of neutralizing antibodies for WNV in free-ranging individuals of abundant passerine species suggests that birds commonly found in the region may have been involved as amplifying hosts in local transmission cycles of WNV.
Introduction: In Brazil, West Nile virus (WNV) was first detected, in 2018, in horses with neurological disease. Aim: We report the first case of WNV infection in a horse from Ceará state and the complete genome sequence of an isolate from Espírito Santo state. Both infections occurred in 2019. Methods: WNV was isolated from the tissues of a horse with neurological signs in Espírito Santo and sequenced by MiSeq. Results: Phylogenetic analysis revealed that the isolate belongs to lineage 1a, clustering with the NY99 strain, a strain that has not circulated in the USA since 2005. Conclusions: Our findings reinforce the hypothesis that WNV has been silently circulating in Brazil for many years.
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