The TAC CV% seems to be a useful and superior marker, compared with SD alone, for assessing medication nonadherence and the possibility of allograft rejection in pediatric renal transplantation.

In the radiation belts, energetic and relativistic electron precipitation into the atmosphere is expected to be mainly controlled over the long term by quasilinear pitch‐angle scattering by whistler‐mode and electromagnetic ion cyclotron waves. Accordingly, statistical electron lifetimes have been derived from quasilinear diffusion theory on the basis of multi‐year wave statistics. However, the full consistency of such statistical quasilinear models of electron lifetimes with both measured electron lifetimes, spectra of trapped and precipitated electron fluxes, and wave‐driven diffusion rates inferred from electron flux measurements, has not yet been verified in detail. In the present study, we use data from Electron Loss and Fields Investigation (ELFIN) mission CubeSats, launched in September 2018 in low Earth orbit, to carry out such comparisons between quasi‐linear diffusion theory and observed electron flux variations. We show that statistical theoretical lifetime models are in reasonable agreement with electron pitch‐angle diffusion rates inferred from the precipitated to trapped 100 keV electron flux ratio measured by ELFIN after correction for atmospheric backscatter, as well as with timescales of trapped electron flux decay independently measured over several days by ELFIN. The present results demonstrate for the first time a broad consistency between timescales of trapped electron flux decay, the pitch‐angle distribution of precipitated electrons, and quasilinear models of wave‐driven electron loss, showing the reliability of such statistical electron lifetime models parameterized by geomagnetic activity for evaluating electron precipitation into the atmosphere during not too disturbed periods.

In biological systems and nanoscale assemblies, the self-association of DNA is typically studied and applied in the context of the evolved or directed design of base sequences that give complementary pairing, duplex formation, and specific structural motifs. Here we consider the collective behavior of DNA solutions in the distinctly different regime where DNA base sequences are chosen at random or with varying degrees of randomness. We show that in solutions of completely random sequences, corresponding to a remarkably large number of different molecules, e.g., approximately 10 12 for random 20-mers, complementary still emerges and, for a narrow range of oligomer lengths, produces a subtle hierarchical sequence of structured self-assembly and organization into liquid crystal (LC) phases. This ordering follows from the kinetic arrest of oligomer association into long-lived partially paired double helices, followed by reversible association of these pairs into linear aggregates that in turn condense into LC domains.T he selectivity and reversibility of DNA and RNA association enables crucial biological functions in which oligomers selectively pair to target sequences even within large amounts of nucleic acid chains. Selectivity is decisive, for example, in the microRNA-mRNA interactions, crucial in the regulation of gene expression. Similar high levels of selectivity are exploited in genomic PCR, relying on the capacity of primers to target their complementary sequence within a full genome. Selective interactions of DNA oligomers have been exploited in the past years in a variety of strategies for the construction of designed self-assembled nanostructures (1-4). Selectivity combines with self-assembly in the recent observation that short oligomers of nucleic acids having complementary sequences exhibit liquid crystal (LC) ordering (5-7). In this article, we report LC ordering in solutions of DNA oligomers with random sequences where the large body of different competing sequences effectively reduces the selectivity of the interactions. With these results, we show that the phenomenology of the self-assembly of nucleic acid oligomers is actually much richer than previously recognized, involving self-selection, linear aggregation, and ordering of fully random chains. Our results strengthen the notion that DNA and RNA have unequaled capacity of self-structuring and unavoidably suggests self-assembly as the possible key factor for the emergence of nucleic acids from the prebiotic molecular clutter as the coding molecules of life. LC Ordering of Complementary DNA SequencesThe first observations of LC ordering of oligonucleotides were performed in solutions of 6-to 20-base-pair DNA oligomers (6 bp ≤ N B ≤ 20 bp) whose sequences promoted the formation of fully paired duplexes (example 1 in Fig. 1A). These were found to order into the chiral nematic (N Ã ) LC phase in concentration (c DNA ) ranges depending on the oligomer length and sequence. At larger c DNA , the solutions transform into the columnar (COL) phase and, at ...

This study shows a significant 4.5-fold risk of graft failure at two years postbiopsy with presence of > or =2 CD20+ cells/hpf. Moreover, > or =3 CD20+ lymphocytes were highly associated with acute cellular rejection. They may be functioning as professional antigen-presenting cells in the graft. In steroid-refractory cellular rejections, therapies that target B cells may prolong graft survival.

No abstract

The Electron Loss and Fields Investigation with a Spatio-Temporal Ambiguity-Resolving option (ELFIN-STAR, or heretoforth simply: ELFIN) mission comprises two identical 3-Unit (3U) CubeSats on a polar (∼93∘ inclination), nearly circular, low-Earth (∼450 km altitude) orbit. Launched on September 15, 2018, ELFIN is expected to have a >2.5 year lifetime. Its primary science objective is to resolve the mechanism of storm-time relativistic electron precipitation, for which electromagnetic ion cyclotron (EMIC) waves are a prime candidate. From its ionospheric vantage point, ELFIN uses its unique pitch-angle-resolving capability to determine whether measured relativistic electron pitch-angle and energy spectra within the loss cone bear the characteristic signatures of scattering by EMIC waves or whether such scattering may be due to other processes. Pairing identical ELFIN satellites with slowly-variable along-track separation allows disambiguation of spatial and temporal evolution of the precipitation over minutes-to-tens-of-minutes timescales, faster than the orbit period of a single low-altitude satellite (Torbit ∼ 90 min). Each satellite carries an energetic particle detector for electrons (EPDE) that measures 50 keV to 5 MeV electrons with $\Delta $ Δ E/E < 40% and a fluxgate magnetometer (FGM) on a ∼72 cm boom that measures magnetic field waves (e.g., EMIC waves) in the range from DC to 5 Hz Nyquist (nominally) with <0.3 nT/sqrt(Hz) noise at 1 Hz. The spinning satellites (Tspin$\,\sim $ ∼ 3 s) are equipped with magnetorquers (air coils) that permit spin-up or -down and reorientation maneuvers. Using those, the spin axis is placed normal to the orbit plane (nominally), allowing full pitch-angle resolution twice per spin. An energetic particle detector for ions (EPDI) measures 250 keV – 5 MeV ions, addressing secondary science. Funded initially by CalSpace and the University Nanosat Program, ELFIN was selected for flight with joint support from NSF and NASA between 2014 and 2018 and launched by the ELaNa XVIII program on a Delta II rocket (with IceSatII as the primary). Mission operations are currently funded by NASA. Working under experienced UCLA mentors, with advice from The Aerospace Corporation and NASA personnel, more than 250 undergraduates have matured the ELFIN implementation strategy; developed the instruments, satellite, and ground systems and operate the two satellites. ELFIN’s already high potential for cutting-edge science return is compounded by concurrent equatorial Heliophysics missions (THEMIS, Arase, Van Allen Probes, MMS) and ground stations. ELFIN’s integrated data analysis approach, rapid dissemination strategies via the SPace Environment Data Analysis System (SPEDAS), and data coordination with the Heliophysics/Geospace System Observatory (H/GSO) optimize science yield, enabling the widest community benefits. Several storm-time events have already been captured and are presented herein to demonstrate ELFIN’s data analysis methods and potential. These form the basis of on-going studies to resolve the primary mission science objective. Broad energy precipitation events, precipitation bands, and microbursts, clearly seen both at dawn and dusk, extend from tens of keV to >1 MeV. This broad energy range of precipitation indicates that multiple waves are providing scattering concurrently. Many observed events show significant backscattered fluxes, which in the past were hard to resolve by equatorial spacecraft or non-pitch-angle-resolving ionospheric missions. These observations suggest that the ionosphere plays a significant role in modifying magnetospheric electron fluxes and wave-particle interactions. Routine data captures starting in February 2020 and lasting for at least another year, approximately the remainder of the mission lifetime, are expected to provide a very rich dataset to address questions even beyond the primary mission science objective.

In pediatric renal transplantation, sirolimus and tacrolimus CV % is a potential tool for monitoring patients at risk for allograft rejection and donor-specific antibodies secondary to medication nonadherence.

A new liquid crystal phase, denoted modulated helical nanofilament (HNF(mod)), is formed from a very simple class of biphenyl carboxylates lacking the benzylidene aniline moieties typically found in HNF mesogens. The HNF(mod) phase represents a novel kind of nanoparticle possessing stacked aromatic rings, with potential applications in organic electronics.