No abstract

he objectives of this paper are to evaluate the addition of nanocobalt on growth of cellulolytic bacteria, degradability of dry matter, cellulose and hemicellulose regarding In vitro gas production. The different levels of nanocobalt as addition element on fermentation kinetics were 0, 25,50,75,100 and 125 % from requirements of animal in ration as dry matter base. Cellulolytic bacteria had been isolated :Cellulomonas cellulasea, Acetobacter xylinum, Thermonospora fusca, Ruminococcus albus , Bacillus sp., Clostridium cellulovorans and Selenomonas ruminantium. Standard ration (1:1 concentrate :clover hay) was incubated for 48 hours. The results indicated that the values of DMD , Hemi.D and Cellul.D, which nanocobalt addition were higher when levels were 50 and 75% compared control. Total cellulolytic bacteria counts in rumen content were higher (P<0.05) in cobalt 100% (11.30) and nanocobalt 75,100 and 125% (11.73,12.72 and 12.20), respectively, while lower value was in nanocobalt 25% (9.72) compared control (7.58). Results revealed that added nanocobalt affect on growth of cellulolytic bacteria and increase degradability of cellulose. The nanocobalt additives had no effect on ruminal pH but more effect on ruminal ammonia and TVF's values, as well as degradability of cell wall constituents and microbial protein production. The extent of gas production was high in control ration , adding cobalt (100 %) and nanocobalt (50%) than other adding levels. Microbial protein (M P) and efficiency microbial protein (EM P) recorded higher values in all different(25,50,75,100 and 125%) added of nanocobalt compared to control (0%). Gas production degradability after 48 hours incubation hemicellulose of 25% nanocobalt recorded the highest value (2444.69) compared to any adding nanocobalt levels and control, while degradability for cellulose recorded higher values for 50 % nanocobalt compared to other levels and control. It concluded that adding nanocobalt improved gas production, growth of cellulolytic bacteria ,ammonia ,total volatile fatty acids, metabolisable energy and cell wall constituents degradability.

This paper focuses on treated date seed with two cellulolytic bacteria (Acetobacter xylinum and Thermonospora fusca) isolated from sheep and evaluated these species by Invitro gas production and metabolism trail. We evaluated the influence of many rations contain several percentage from date seed untreated and treated on In vitro traild for DM,OM, NDF, ADF, cellulose and hemicellulose disappeara (samples incubated for 24 hrs). the best ration used in metabolism trail .