The newest achievements in the application of adsorption and reversed‐phase high‐performance liquid chromatography (HPLC) used for the separation and quantitative determination of various pesticides is discussed in detail. A short overview of the basic principles of the technique is given, and the main theoretical equations employed for the characterization of retention processes and the quality of separation are presented. Instrumentation and troubleshooting are outlined to the extent to give a working knowledge for the solution of practical separation problems for the analysts. The sample preparation strategies such as liquid–liquid, supercritical fluid, solid‐phase and solid‐phase microextraction suitable for the enrichment, and prepurification of pesticides in complicated matrices are surveyed. The techniques employed for the measurement of pesticides classes such as organochlorine, organophosphorous, carbamate, urea, triazine, phenoxyalkyl acid, and pyrethroid pesticides are compiled and critically evaluated. The newest and the most adequate methods are described in detail, whereas the previous HPLC procedures are shortly referred.
High‐performance liquid chromatography (HPLC) has been developed as a rapid and sensitive method for the separation and quantitative determination of a wide variety of compounds present in complex matrices in very low concentration. As gas–solid and gas–liquid chromatographic techniques are only suitable for the analysis of volatile and thermostable compounds with relatively low molecular mass, HPLC methods can also separate nonvolatile, thermolabile, and large molecules. The excellent separating power, high sensitivity, and considerable diversity of solutes that could be separated with HPLC contributed to its popularity.
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