Background: Colistin is the last choice for serious infections caused by multidrug-resistant Gram negative bacteria and one of the prominent causes for spreading the resistance is Plasmid-borne Mobile Colistin Resistance (mcr). Broth microdilution method (BMD) is the reference tool for colistin minimum inhibitory concentrations (MIC) determination, but it has many obstacles, so commercial BMD methods had been developed that are more userfriendly than the reference method and (Liofilchem ® ComASPTM) is one of them, which we used to determine colistin MIC in this study. Objective: To detect colistin resistant Gram negative bacilli (GNB) by ComASPTM colistin (formerly Sensi Test™Colistin) among Intensive Care Units (ICUs) patients admitted to Ain Shams university hospitals and to screen the presence of mcr-1 gene by Polymerase Chain Reaction (PCR) in Colistin resistant isolates. Method: This Observational cross-sectional study was performed in the
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