The twenty-two bacterial isolates were screened for cellulolytic activity using Congo red stain on Carboxymethylcellulose (CMC) agar plates inoculated with the isolates. All the isolates were found to hydrolyze Carboxymethylcellulose. Five bacterial isolates (FAY88, FAY103, FAY117. FAY136 and FAY182) were selected according to their higher production of carboxymethyl cellulase (CMCase). A Gram stain test was carried out to identify five isolates as Gram-positive rods, all associated mainly with members of the Bacillus. Their CMCase activities were 1.49, 1.26, 1.21, 1.21 and 1.24 U/ml, respectively. Genetic distances for the five selected strains with high cellulase activity based on the 79 bands obtained from the results of RAPD revealed the similarity according to the morphological characterization agrees with genetic distance. The SDS-PAGE analysis was revealed that, the molecular weight of crude cellulase isolated from Bacillus isolates was found approximately of 54 kDa.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.