The application of the hydroperoxidase activity of immobilized lipoxygenase to xenobiotic metabolization is reported in this work. Soya bean lipoxygenase has been immobilized by covalent coupling to oxirane acrylic beads. This immobilized system has been applied to the oxidation of chlorpromazine (CPZ), a phenothiazine of wide pharmacological use which in some cases presents toxicity. Immobilized lipoxygenase produces the oxidation of CPZ in the presence of hydrogen peroxide at acidic pH, maintaining a high level of activity and stability. In comparison with free lipoxygenase, the immobilized enzyme system shows higher catalytic efficiency and protection against enzymic inactivation produced by the presence of H(2)O(2). When the system of immobilized enzyme was loaded into a bioreactor operating in continuous mode, the level of CPZ oxidation was higher than obtained using a discontinuous procedure or the free enzyme. The results obtained in this work suggest that a system of covalent immobilized lipoxygenase operating in continuous mode may constitute a valuable tool for xenobiotic detoxification or metabolization studies.
La dieta mediterránea es considerada un modelo de alimentación saludable debido a los múltiples beneficios que representa. El objetivo fue determinar la influencia del lugar de residencia en el patrón dietético, así como la adherencia a la dieta mediterránea en estudiantes de Cáceres. El estudio fue llevado a cabo en 171 estudiantes del grado de enfermería de la Facultad de Enfermería y Terapia Ocupacional de Cáceres. Tanto el lugar de residencia como la adherencia a la dieta mediterránea se establecieron utilizando el índice Kidmed. El 75.4% de los estudiantes que vivían en su residencia familiar consumía una pieza de fruta todos los días, mientras que solo el 48.2% de los estudiantes universitarios que compartían apartamento lo hacía. La ingesta diaria de más de una fruta y hortalizas frescas o cocidas fue similar en ambos grupos. Se observaron diferencias significativas en la puntuación total del índice Kidmed entre los grupos. Los estudiantes que viven en la residencia de su familia consumen más frutas y verduras, lo que resulta en un estilo de vida más saludable. El nivel de adherencia a la dieta mediterránea fue similar en ambos grupos. Sin embargo, se observaron puntuaciones totales más bajas en el grupo de estudiantes que compartían apartamento.
This work describes the application of the N-demethylase activity of immobilized soybean lipoxygenase to the oxidative degradation of xenobiotics. Previously (1) we have shown that immobilized lipoxygenase produces the oxidative degradation of CPZ in the presence of hydrogen peroxide. As a continuation of this work, here we studied the N-demethylation of CPZ by the hydroperoxidase activity of covalent immobilized soybean lipoxygenase. The obtained results clearly reveal that the immobilized system produces the N-demethylation of CPZ in the presence of hydrogen peroxide, maintaining a high level of activity in comparison with free enzyme. Additionally, the immobilized lipoxygenase shows stability higher than that of free enzyme, making feasible its use in a bioreactor operating in continuous or discontinuous mode. The results obtained in this work, together with those obtained previously by us for the oxidation of CPZ, suggest that hydroperoxidase activity of immobilized lipoxygenase may constitute a valuable tool for oxidative xenobiotics degradation or for application to synthetic processes in which a N-demethylation reaction is involved.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.