Matricaria chamomilla L. is a popular medicinal herb that is used for healing various diseases and is widely distributed worldwide in temperate climate zones, and even in the subtropical climate of Southern and Western Iran. This study was aimed at comparing the volatile oil constituents, along with antiradical potential and HPLC analysis of methanolic extracts from twelve plant samples growing in Iran. The present research was carried out for the first time on these populations. Among seventeen identified volatile chemicals evaluated by GC/MS and GC/FID, representing 92.73–97.71% of the total oils, α-bisabolone oxide A (45.64–65.41%) was the major constituent, except in case of “Sarableh” as a new chemotype, where (E)- and (Z)-γ-bisabolene (42.76 and 40.08%, respectively) were the predominant components. Oxygenated sesquiterpenes (53.31–74.52%) were the most abundant compounds in the samples excluding “Sarableh” with 91.3% sesquiterpene hydrocarbons. “Sarableh” also exerted the most potent antioxidant capacity with EC50 = 7.76 ± 0.3 µg/mL and 6.51 ± 0.63 mmol TE (Trolox® equivalents)/g. In addition, populations “Lali” and “Bagh Malek” contained the highest amounts of apigenin and luteolin with 1.19 ± 0.01 mg/g and 2.20 ± 0.0 mg/g of plant material, respectively. Our findings depict a clear correlation between phytochemical profiles and antiradical potential of M. chamomilla and geographical factors.
We studied 200 trees belonging to 20 accessions of cultivated olive (O. europaea L.) from 4 regions of origin, evaluated by means of agromorphological traits and simple sequence repeat (SSR) markers. The agromorphological traits showed high variation between genotypes and significant correlation coefficients were obtained among the values recorded in two consecutive years, 2013 and 2014. The maximum coefficient of variation for the quantitative agronomic traits was observed in fruit weight wet (13.45%), while the lowest was found in stone width (3.18%). Fruit shape index, leaf length, leaf width, and lenticel size also showed variability. With both DNA-based and agromorphological descriptors, higher levels of variability were found. Genetic variation observed among the olive germplasm at the DNA level was higher than that of the agromorphological traits, indicating the efficiency of SSR markers for detecting genetic diversity among olive genotypes and their relationships. The lack of consistency between the relationship studies performed with molecular and morphological markers could indicate that each marker system measures different aspects of olive genetic variability. Molecular data obtained by SSR markers together with morphological and agronomical characterization of olive trees confirmed the high diversity and their potential use for olive breeding.
Currently, study of the inter and the intra-population genetic disparity was done by use of the 200 Olea europaea L. which is found growing naturally in the nation of Iran, and this study was carried out by AFLP and IRAP markers. The fingerprints that were similar to the AFLP and the IRAP markers were evidence of high concentrations of heterozygosity and this shows that O. europaea L. is primarily the out crossing species. The average percentage of polymorphism is as shown below: 87.15 and 87.38% of the information used in regard to the AFLP and the IRAP, respectively. The gene disparity numerals on the population researched were 1.087 for HT and 0.871 for HS in regard to AFLP. For the IRAP it was 1.084 for HT and 0.860 for HS. The general values for genetic variations that are found in the O. europaea L. germplasm in the nation of Iran were then assessed through putting together the AFLP and the IRAP information so as to cover a larger genome. Arguing from the AFLP and the IRAP studies, it can be concluded that there are more levels of genetic variation at inter and the intra-population level for the O. europaea.
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