A novel coordinated power controller design framework is proposed to optimize the active power output of multiple generators in a distributed network. Each bus in the distributed generation systems includes two function modules: a distributed economic dispatch module and a cooperative control module. By virtue of the distributed consensus theory, a distributed economic dispatch algorithm is proposed and utilized to calculate the optimal active power generation references for each generator. The cooperative control module receives and tracks the active power generation references such that the generation-demand balance is guaranteed at minimum operating cost while satisfying all generation constraints. The distributed control and management strategies enhance the redundancy and the plug-and-play capability in microgrids. Optimal properties and convergence rates of the proposed distributed algorithms are strictly proved. Simulation studies further demonstrate the effectiveness of the proposed approach.
Cysteine proteinases (Cyps) play vital roles in many biological processes, including physiological and pathological reactions. In the present study, we cloned a full cDNA of SlCyp, encoding a 344-amino-acid protein from Spodoptera litura. The putative amino acid sequence shared >75% identity with Cyps from other insects. A phylogenetic analysis revealed that SlCyp is closely related to other known lepidopteran Cyps. Real-time PCR and Western blotting analyses showed that SlCyp is induced by Nomuraea rileyi infection in all the tissues tested. The strongest SlCyp mRNA and protein expression was found in haemocytes, followed by the fat bodies, of unchallenged and N. rileyi-challenged S. litura. A time-course analysis showed that SlCyp mRNA and protein expression levels were upregulated in the haemocytes and fat bodies by N. rileyi infection. Upon N. rileyi infection, the proteolytic activities of SlCyp were also significantly higher in the haemolymph than in normal or phosphate-buffered-saline-challenged controls. These results suggest that SlCyp plays an important role in the innate immunity of S. litura in response to N. rileyi. SlCyp mRNA and protein expression and activities were also elevated during sixth-instar moulting and metamorphosis. Knocking down SlCyp transcripts with double-stranded RNA interference caused prepupal, pupal, and adult phenotypic changes, and SlCyp-silenced mutant larvae displayed a significantly lower survival rate after N. rileyi infection. These facts suggest that SlCyp plays a significant role in resisting N. rileyi infection and an essential role in larval development. Our data should facilitate the development of techniques for S. litura control.
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