Thioredoxin peroxidase gene is involved in resistance to biocontrol fungus Nomuraea rileyi in Spodoptera litura: Gene cloning, expression, localization and function

Chen, Huan; Yin, Yue; Feng, Eryan; Li, Yan; Xie, Xiang; Wang, Zhongkang

doi:10.1016/j.dci.2013.11.012

Cited by 22 publications

(13 citation statements)

References 41 publications

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“…In addition, the ROS generated gets scavenged by the low-molecular-weight free-radical scavengers and defensive enzymes including PPO, POD, CAT, and SOD (Qin, Lu, Zheng, & Du, 2016). These enzymes are the determiners of oxidative resistance under adverse environmental stress conditions (Chen et al, 2014;Ma et al, 2017;Paithankar, Raghu, & Patil, 2018). Thus, a study to understand the role of entomic antioxidants in resistance to pesticides could be helpful.…”

Section: Discussionmentioning

confidence: 99%

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Zeng

Shi

Guo

et al. 2020

Arch Insect Biochem Physiol

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To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After feeding on the diets with different pH values for 84 hr, fourth‐instar caterpillars were randomly collected. Growth and various physiological traits were determined and 16S recombinant DNA sequencing was performed using the intestinal microflora of surviving larvae. Results showed that the mortality was 30% in DPB6, and 10% in DPB8, while no mortality was observed in control. The partial least squares discriminant analyses suggested that diets prepared with PB of different pH resulted in different food intake, amount of produced feces, weight gain, digestive enzyme activities, and antioxidant enzyme activities in larvae. Interestingly, both the highest weight gain and the lowest total antioxidant capacities were seen in control larvae. Results also showed that the larval gut microbiota community structure was significantly affected by dietary pH. Moreover, linear discriminant analysis effect size suggested that the family Acetobacteraceae in control, genus Prevotella in DPB8, and genus Lactococcus, family Flavobacteriaceae, family Mitochondria, and family Burkholderiaceae in DPB6 contributed to the diversity of the larval gut microbial community.

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Section: Discussionmentioning

confidence: 99%

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Zeng

Shi

Guo

et al. 2020

Arch Insect Biochem Physiol

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“…In insects, several Prx genes have been identified in Drosophila melanogaster , Spodoptera litura , Apis cerana cerana and Bombus ignitus (Chen et al ., ; Hu et al ., ; Kang et al ., ; Michalak et al ., ; Yu et al ., ). The silkworm, Bombyx mori , is a key model system for lepidopteran genomics and genetics studies.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of an atypical 2‐cys peroxiredoxin from the silkworm, Bombyx mori

Wang

Zhou

Chen

et al. 2016

Insect Molecular Biology

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Peroxiredoxins (Prxs) play an important role in the protection of insects against the toxicity of reactive oxygen species. Here, we identified and characterized a novel, atypical 2-cysteine (Cys) peroxiredoxin (BmPrx3) from an expressed sequence tag database in a lepidopteran insect, Bombyx mori. The BmPrx3 cDNA contained an open reading frame of 684 bp that encodes a 228-amino-acid protein with a calculated molecular mass of 25 kDa. Sequence comparison revealed that BmPrx3 belongs to the atypical 2-Cys Prxs. Quantitative real-time PCR revealed that BmPrx3 can be detected in all tissues and developmental stages. Recombinant BmPrx3 purified from Escherichia coli exhibited antioxidant activity that removed hydrogen peroxide and protected DNA from oxidative damage. Disc diffusion and viability assays revealed that recombinant BmPrx3 increased bacterial survival under H2 O2 -mediated oxidative stress. In addition, quantitative real-time PCR analysis indicated that BmPrx3 transcription levels were significantly increased in response to various oxidative stresses. Furthermore, BmPrx3 transcription levels in the midgut were regulated by bacterial infection. Taken together, these results suggest that BmPrx3 acts as an antioxidant enzyme to protect the silkworm from various oxidative stresses.

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“…They are indispensable enzymes for insects dealing with exogenous substance (Aranda et al, 2014;Oakley, 2011;Wang et al, 2018). Antioxidant enzymes, which play critical roles in dealing with adverse environmental stress, are determiners of oxidative resistance (H. Chen et al, 2014;Ma et al, 2017;Paithankar, Raghu, & Patil, 2018). Investigations about entomic antioxidase would be helpful for preventing the development of resistance to pesticides.…”

Section: Introductionmentioning

confidence: 99%

Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

Zeng

Shi

et al. 2019

Arch Insect Biochem Physiol

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To understand how ambient temperature affect the gypsy moth larvae, and provide a theoretical basis for pest control in different environments. Fourth instar gypsy moth larvae were incubating for 3 hr at 15℃, 20℃, 25℃, 30℃, 35℃, and 40℃, respectively. Afterward, digestive and antioxidant enzyme activities, total antioxidant capacity, and intestinal microflora community were analyzed to reveal how the caterpillars respond to ambient temperature stress. Results showed that both digestive and antioxidant enzymes were regulated by the ambient temperature. The optimum incubation temperatures of protease, amylase, trehalase, and lipase in gypsy moth larvae were 30℃, 25℃, and 20℃, respectively. When the incubation temperature was deviated optimum temperatures, digestive enzyme activities would be downregulated depending on the extent of temperature stress. In addition, glutathione S‐transferase, peroxidase, catalase, and polyphenol oxidase would be activated under a sufferable temperature stress, but superoxide dismutase and carboxylesterase (CarE) would be inhibited. In addition, results showed that the top two abundant phyla were Proteobacteria and Firmicutes. The phylum Firmicutes abundance was decreased and phylum Proteobacteria abundance was increased by ambient temperature stress. Moreover, it suggested that gypsy moth caterpillars at different ambient temperature mainly differed from each other by Escherichia‐Shigella and Bifidobacterium in control, Acinetobacter in T15, and Lactobacillus in T40, respectively.

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Thioredoxin peroxidase gene is involved in resistance to biocontrol fungus Nomuraea rileyi in Spodoptera litura: Gene cloning, expression, localization and function

Cited by 22 publications

References 41 publications

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

Identification and characterization of an atypical 2‐cys peroxiredoxin from the silkworm, Bombyx mori

Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

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