Background and Aim: Studies to determine abundance, distribution, species composition, and mosquito interactions are very important in understanding the risk of disease transmission to implement appropriate mosquito management in endemic areas. Lymphatic filarial worms are one of the parasites that are contracted and/or transmitted by mosquitoes when sucking the blood of infected humans or animals and then biting others. This research was conducted to study the abundance, species composition, mosquito biting cycles, density and periodicity of mosquitoes caught in Lubuk Pauh Village, Bulang Tengah Suku Ulu, Musi Rawas, South Sumatera, Indonesia, which is an endemic area of zoonotic Brugia malayi. Materials and Methods: The mosquito collection was done in July 2018 using the human landing collection method for 11 h from 18.00 pm to 5.00 am Western Indonesian Time. The catching of mosquitoes was done both indoors and outdoors, and mosquitoes were identified under a dissecting microscope using an identification key to confirm their species. Detection of B. malayi larvae in mosquitoes was confirmed by dissection and polymerase chain reaction methods. Results: The caught mosquitoes consisted of four species: Armigeres subalbatus, Culex quinquefasciatus, Culex vishnui, and Mansonia uniformis. Based on the Shannon–Wiener index, Lubuk Pauh Village has low mosquito species diversity (0.210). Ar. subalbatus was the dominant mosquito in Lubuk Pauh Village with dominance number 95.08, and it had the most frequent activity in each of periods of indoor and outdoor collection, with the highest density (man-hour density) at 18.00-19.00 (51.750). B. malayi infective stage larvae were not found in all mosquito species caught. Conclusion: Existence of Ar. subalbatus, Cx. quinquefasciatus, and Ma. uniformis in Lubuk Pauh Village which is an endemic area of B. malayi shows that the area is at risk of lymphatic filariasis transmission.
Background and Aim: Brugia malayi is known to be zoonotically important because it can be transmitted from animals (mammals and primates) to humans or from humans to humans through mosquito vectors. This study was conducted to explore the fauna associated with Malayan filariasis transmission in Sedang village, Suak Tapeh District, Banyuasin Regency, South Sumatra Province, Indonesia. Materials and Methods: A cross-sectional research design with an observational and analytical approach was applied in this study, and it was conducted in May 2018. Mosquitoes were collected twice using human bait both inside and outside the house from 6:00 p.m. to 6:00 a.m. The presence of competitors, predators, and reservoir hosts in the areas of five breeding habitats of Mansonia spp. was observed. The presence of microfilaria was confirmed under a microscope in night blood samples of inhabitants and cats. The presence of infective larvae (L3) of B. malayi was identified microscopically and based on the polymerase chain reaction method in female Mansonia mosquitoes. Results: A total of 12 mosquito species were found, among which Mansonia uniformis was the dominant mosquito, and the predominant competitor was Mansonia annulifera. Dragonflies, as predators were found in two breeding habitats and fish were found in one breeding habitat. The L3 of B. malayi were not identified in the mosquitoes, and the microfilariae of B. malayi were not found in the blood samples of inhabitants and cats. Conclusion: Although Mansonia mosquito population was abundant in Banyuasin Regency, the mosquito was not confirmed as an intermediate host of B. malayi, and the cat was not confirmed as a reservoir of B. malayi in the location.
Background: Lymphatic filariasis is a parasitic disease that is similar to the threads of its habitat in the lymph system that infect humans, namely Wuchereria bancrofti, Brugia malayi, and Brugia timori. Although B. malayi commonly infects humans, recent evidence also suggests that Brugia pahangi, an afilarial nematode naturally found in cats, can cause clinical infection in humans, with clinical features consistent with lymphatic filariasis. Methods: Cross-sectional with an observational and analytic approach. The results of a positive microscopic examination were carried out by Brugia Rapid Test for B. malayi and PCR examination for B. malayi and B. pahangi. Positive microscopic results were then checked for periodicity of microfilariae every 2 hours for 24 hoursResults: From the research, 17 people were positive for B. malayi microfilariae (mf rate 6.34%). The Brugia Rapid Test had 17 positive results. PCR results of 14 people were positive/formed a band at 322 bp. The results of the sample sequencing were B. malayi species. PCR results of B. pahangi were not found to be positive / band formed in all samples. The periodicity results of microfilaria peaked at 00: 00-04: 00 with the nocturnal periodic type. Conclusions: Lubuk Pauh Village, Musi Rawas Regency is still endemic for malayi filariasis with a high level of endemicity and is not an endemic area for filariasis pahangi. The periodicity of microfilariae indicates a nocturnal periodic type.
Abstrak Pemeriksaan lemak darah seperti kolesterol merupakan salah satu parameter kimia klinik yang berguna untuk mengetahui ada tidaknya peningkatan kadar kolesterol dalam tubuh. Pemeriksaan lemak darah dilakukan dengan mengunakan sampel serum atau plasma. Spesimen yang biasa digunakan sampel serum atau plasma dengan cara dicentifuge dengan kecepatan 3000 RPM selama 5-15 menit. Kenyataan di lapangan sering kali diabaikan oleh beberapa teknisi dengan mengurangi lama waktu sentrifugasi yang bertujuan untuk mempersingkat waktu pemeriksaan. Sampel darah yang disentrifugasi dengan waktu yang tidak tepat akan merusak enzim lipoprotein pada kolesterol. Waktu sentrifugasi yang terlalu singkat akan menyebabkan serum dan zat-zat yang terkandung didalamnya tidak terpisah sempurna dari sel-sel darah sehingga akan menyebabkan hasil rendah palsu, sementara itu waktu sentrifugasi yang terlalu lama selain dapat merusak senyawa lipoprotein juga akan menyebabkan sampel hemolisis. Tujuan Penelitian: untuk mengetahui hasil analisis pemeriksaan kadar kolesterol menurut lama waktu serum sentrifugasi. Metode Penelitian: Penelitian ini bersifat analitik dengan pendekatan cross-sectional dan dilakukan di Laboratorium Kimia Klinik Jurusan Analis Kesehatan Poltekkes Kemenkes Palembang. Sampel penelitian ini adalah sebanyak 30 sampel. Analisis data menggunakan One Way Anova. Hasil Penelitian: menunjukkan bahwa adanya perbedaan terhadap kadar kolesterol berdasarkan waktu sentrifugasi dengan p value 0.004 (p<ɑ 0.05) dengan nilai rata-rata hasil pemeriksaan kadar kolesterol yang disentrifugasi selama 5 menit sebesar 180.90 mg/dL, selama 10 menit sebesar 158.73 mg/dL, selama 15 menit sebesar 169.60 mg/dL. Kesimpulan: Adanya perbedaan terhadap kadar kolesterol berdasarkan waktu Sentrifugasi. Kata Kunci : kolesterol, waktu sentrifugasi, kecepatan sentrifugasi
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.