No abstract
Recent advances are nowadays providing opportunities to examine the complexities of organs and organisms at the single-cell level. The conventional cell.based analysis mainly examines the cellular processes from the bulk of cells but single.cell omics provides a more detailed insight into individual cell phenotypes, thus giving a link between the phenotype and genotype of cells. Single-cell analysis can be performed at genome, epigenome, transcriptome, proteome and metabolome levels and thus makes it possible to come across mechanisms not seen during the sequencing of bulk tissues. Researchers need to isolate single cells before the initiation of single-cell analysis. For this, various strategies like FACS, MACS, LCM, micro-manipulation and micro-fluids are used for cell isolation depending upon their physical properties and cellular biological characteristics. The analysis of single-cell data at multiple levels gives us an unusual view of multilevel transformation at the single-cell level and thus providing a better chance to discover novel biological processes. High throughput analysis of single cells at genome, transcriptome and proteome levels provides unique and important insights into cell variability and diverse processes like development, genetic expressions and severity of different symptoms in disease pathogenesis.
The potato (Solanum tuberosum L.) plant is grown in about 150 countries of the world and is considered an important food crop. However, this crop is susceptible to different biotic and abiotic factors, which can affect its crop yield. This vulnerability can be reduced or eliminated by growing potatoes under sterilized conditions. Cytokinins, such as 6-Benzylaminopurine, are proven to show a significant role in the in vitro regulation of plants. In the current study, explants of Kuroda variety were/potato cv. Kuroda were grown using diverse concentrations of 6-Benzylaminopurine (BAP), which displayed varied results. BAP concentration of 0.01 mg/l showed a 10 cm shoot length with 41 shoots having 66.66% regeneration efficiency. Meanwhile, the explant grown in 0.25 mg/l BAP concentration showed 16 cm shoot length with 65 shoots having 83.33% regeneration efficiency. On the other hand, the explants that were grown using 0.05 mg/l and 1 mg/l BAP concentration showed 7 cm and 10 cm shoot length with 35 and 52 shoots having 63.33% and 76.66% regeneration efficiency, respectively. Therefore, it was concluded that 0.25 mg/L of BAP showed the best results with the highest number of shoots and shoot length as well as maximum regeneration efficiency among all the tested concentrations.
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