Febriyani E, Falah S, Andrianto D, Lastini T. 2018. Identification of active compounds and anti-acne activity from extractsand fractions of surian (Toona sinensis) leaves planted in Sumedang, West Java, Indonesia. Biodiversitas 19: 1406-1412. Surian or toon(Toona sinensis Merr) leave is one of the herbs containing many bioactive compounds as potential sources of anti-acne agents. The aimof this work is to identify and evaluate the anti-acne activity of Surian leaves extract and fractions against acne bacteria. Crude extractsof Surian leaves from various solvents (methanol, ethanol, and ethyl acetate) were screened for their antimicrobial activity using discdiffusion. The results showed that ethyl acetate extract demonstrated the highest inhibition against Propionibacterium acne (49.93%),Staphylococcus epidermidis (57.14%), Staphylococcus aureus (18.93%), and Eschericia coli (82.03%). The results of Brine ShrimpLethality Test (BSLT) showed that the methanol extract had the highest cytotoxicity of LC50 at 29.76 μg/mL. The extract was thenfractionated using column chromatography, resulted in 6 fractions. The fraction 3 showed the best antibacterial effect against P. acne(28.27%), S. epidermidis (7.38%) and S. aureus (10.38%). Minimum inhibitory concentration (MIC) and minimal bactericidalconcentration (MBC) test were carried out using liquid dilution, whereas dimethyl sulfoxide (DMSO) and Clindamycin were used as anegative and positive control, respectively. The MIC and MBC test assays of fraction 3 varied for different bacteria P. acne bacteria(5000 and 5000 ppm), S. aureus (1250 and 10000 ppm), E. coli (2500 and 5000 ppm), while for S. epidermidis the fraction 3 was able toinhibit with MIC value of 5000 ppm. Based on microscopic observation using Scanning Electron Microscope (SEM), the fraction 3 atthe dose of 5000 ppm could promote the cellular destruction of P. acne. Further experiment using Liquid Chromatography-TendemMass Spectrometry (LC-MS/MS) showed various compounds associated with the anti-acne activity of the fraction 3, such aspheophorbide a, pheophytin a and dihomo-γ-linolenic acid.
Background : Glucose examination, can use whole blood, serum, and urine samples. Serum was obtained from blood which was awaited to freezing first and then centrifuged at 3000 rpm for 15 minutes. However, the reality in the employment to shorten the time, blood is often centrifuged immediatelly without waiting for the blood to freezing. Purpose : To determine whether there is differences in serum glucose levels from the blood that is immediately centrifuged and waited for freezing before centrifuge. Methods :This research is an analytical research using cross sectional approach. The research sample was taken from 30 students of Medical Laboratory Technology, Poltekkes Palembang. Result :The data obtained were analyzed statistically by using the test t dependet we got the average serum glucose level from blood that is immediately centrifuge was 83,80 mg/dL. The average serum glucose from blood waited for freezing before centrifuge was 76,50 mg/dL. Obtained result P value = 0,000012 means p<0,05. Conclusion : There is statistically meaningful differences beetwen serum glucose levels from the blood that is immediately centrifuge and waited for freezing before centrifuge. Suggestion :it is recommended for laboratory technology should be examined against blood that has waited for freezing before centrifuge, because blood that is immediately centrifuge can increase glucose levels so that the result examination increase falsely.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.