Several adverse health effects, such as respiratory and cardiovascular morbidity, have been linked to exposure to particulate matter in ambient air; however, the biologic activity of gas-phase ambient organic air contaminants has not been examined as thoroughly. Using aryl hydrocarbon receptor (AHR)–based and estrogen receptor (ER)–based cell bioassay systems, we assessed the dioxin-like and estrogenic activities of gas-phase organic ambient air contaminants compared with those of particulate-phase contaminants using samples collected between seasons over 2 years from an urban and a rural location in the Greater Toronto Area, Canada. The concentration of the sum (∑) of polycyclic aromatic hydrocarbons, which was highest in the gas phase, was 10–100 times more abundant than that of ∑polychlorinated biphenyls, ∑nitro-polycyclic aromatic hydrocarbons, and ∑organochlorine pesticides, and 103 to 104 times more abundant than ∑polychlorinated dibenzo-p-dioxins/dibenzofurans. Gas-phase samples induced significant AHR- and ER-dependent gene expression. The activity of the gas-phase samples was greater than that of the particulate-phase samples in the estrogen assay and, in one case, in the AHR assay. We found no strong associations between either summer or winter seasons or urban or rural locations in the relative efficacy of the extracts in either the ER or AHR assay despite differences in chemical composition, concentrations, and abundance. Our results suggest that mechanistic studies of the health effects of ambient air must consider gas and particulate phases because chemicals present in both phases can affect AHR and ER signaling pathways.
A novel environmentally derived mixture that integrates exposure to atmospherically derived gas- and particle-phase compounds in urban areas-namely, the organic film that develops as a thin layer on urban impervious surfaces-was investigated for its ability to induce gene expression via the aryl hydrocarbon receptor (AhR). The organic film on window glass from 21 sites in downtown Toronto (Ontario, Canada) was found to contain a complex mixture of environmental contaminants typical of urban environments, notably PAHs, n-alkanes, PCBs, organochlorine (OC) pesticides, and polar constituents. Using a stably transfected reporter cell line, we found that the crude extract of organic film induces AhR-dependent gene expression in a dose-dependent fashion. Three subfractions of the crude extract induced significant luciferase expression: nonpolar aromatic > polar aromatic > nonpolar aliphatic. Recombination of the fractions did not lead to recovery of the full activity of the crude extract, which may indicate that some of the compounds lost during fractionation were significant contributors to the induction observed with the crude extract. The interactions between a tonic dose of B[ a]P (10(-7) M) and each of the aromatic fractions were determined to be antagonistic following analysis by the method of isoboles. Our results suggest that organic film makes up a diverse array of compounds active at the AhR and that these compounds may not interact in a strictly additive manner.
A mass balance model of contaminant fate-transport was used to assess the fate of four metals: As, Cd, Cu and Zn, in the Bay of Quinte for hydrologic conditions and loadings in 2000. Results were compared with previous model results of 1988. The model was based on the QWASI (Quantitative Water Air Sediment Interaction) approach and the fugacity/aquivalence concept. The Bay was divided into five geographic segments based on hydrodynamics and chemical loadings. The model identified tributaries and Lake Ontario as the major sources of metal loadings to the Upper and Lower Bays, respectively. Metal concentrations in water decreased by 40–75% in the tributaries between May 1988 and 2000, which resulted in decreased metal concentrations that were, in 2000, all below the Provincial Water Quality Objectives. Measured sediment concentrations exceeded the Lowest Effect Levels (LEL) for all metals at many sites in the Upper and Lower Bays. Using 2000 metal loadings from tributaries and Lake Ontario, the model predicted that sediment concentrations will meet or come within 20% of LELs for all metals in segment 1, As in segments 2 and 3, and Zn in segment 3 within 40 years. Although the model predicted that sediment concentrations would decline to the LEL for all segments within 26 (Zn) to 54 (Cu) years, evidence suggests that benthos are now not impaired by ambient sediment concentrations (excluding “hot spots”). Reducing sediment concentrations faster is expected to be very difficult because their loadings originate from diffuse sources in the watersheds of tributaries and Lake Ontario.
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