Key WardsColumn liquid chromatography Indirect enantiomeric separation Chiral derivatization (S)-N-(4-Nitrophenoxycarbonyl)phenylalanine methoxyethyl ester Amino acids
SummaryThe application of (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester, (S)-NIFE, as a new chiral derivatizing agent for the resolution of compounds possessing an amino group is described. Its apphcabihly is demonstrated by the resolution of proteinogenic amino acid enantiomers. The diastereomeric derivatives produced were separated by reversedphase high-performance liquid chromatography. The effects of pH, excess reagent and reaction time on the derivatization kinetics, and the effects of pH and the organic modifier on the separation, were investigated.
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