The
key questions in folding studies are the protein dimensions and the
degree of folding. These properties are best characterized by the
self-diffusion coefficients D determining the hydrodynamic
dimensions. In our present study, we derive empirical variations of D as a function of molecular mass M that
distinguish folded, intrinsically disordered, and urea-denatured biomolecules.
Reliable D values are obtained from diffusion NMR
measurements performed under identical conditions using a representative
set of proteins/peptides with diverse amino acid sequence and length.
The established relations are easy to use analytical tools for molecular
mass analysis and aggregation studies as well. Deriving equations
under denaturing conditions has several pitfalls, and here, we provide
a simple quantitative method for estimating the debated end point
of denaturation, while already the 1D 1H spectrum gives
a qualitative picture of the collapsed, denatured structure. Data
indicate that the intrinsically disordered proteins have a similar
behavior as synthetic polymers and urea-denatured proteins.
Conformationally flexible protein complexes represent a major challenge for structural and dynamical studies. We present herein a method based on a hybrid NMR/MD approach to characterize the complex formed between the disordered p53TAD 1-60 and the metastasis-associated S100A4. Disorder-toorder transitions of both TAD1 and TAD2 subdomains upon interaction is detected. Still, p53TAD 1-60 remains highly flexible in the bound form, with residues L26, M40, and W53 being anchored to identical hydrophobic pockets of the S100A4 monomer chains. In the resulting "fuzzy" complex, the clamplike binding of p53TAD 1-60 relies on specific hydrophobic anchors and on the existence of extended flexible segments. Our results demonstrate that structural and dynamical NMR parameters (cumulative Δδ, SSP, temperature coefficients, relaxation time, hetNOE) combined with MD simulations can be used to build a structural model even if, due to high flexibility, the classical solution structure calculation is not possible.
As part of an International consortium aiming at the characterization by NMR of the proteins of the SARS-CoV-2 virus, we have obtained the virtually complete assignment of the backbone atoms of the non-structural protein nsp9. This small (12 kDa) protein is encoded by ORF1a, binds to RNA and seems to be essential for viral RNA synthesis. The crystal structures of the SARS-CoV-2 protein and other homologues suggest that the protein is dimeric as also confirmed by analytical ultracentrifugation and dynamic light scattering. Our data constitute the prerequisite for further NMR-based characterization, and provide the starting point for the identification of small molecule lead compounds that could interfere with RNA binding and prevent viral replication.
Intracellular organelles do not, as thought for a long time, act in isolation but are dynamically tethered together by entire machines responsible for interorganelle trafficking and positioning. Among the proteins responsible for tethering is the family of VAMP-associated proteins (VAPs) that appear in all eukaryotes and are localized primarily in the endoplasmic reticulum. The major functional role of VAPs is to tether the endoplasmic reticulum with different organelles and regulate lipid metabolism and transport. VAPs have gained increasing attention because of their role in human pathology where they contribute to infections by viruses and bacteria and participate in neurodegeneration. In this review, we discuss the structure, evolution, and functions of VAPs, focusing more specifically on VAP-B for its relationship with amyotrophic lateral sclerosis and other neurodegenerative diseases.
Structural and dynamic characterization of highly flexible protein complexes is a major challenge in structural biology. By a combined NMR spectroscopy and MD simulation approach, we studied the complex formed between the disordered p53TAD1‐60 and the metastasis‐associated S100A4. The results indicate that both subdomains of p53TAD1‐60 undergo a disorder‐to‐order transition, with three residues acting as anchor points attached to the binding pockets of S100A4, and a clamp‐like fuzzy complex is formed. More information can be found in the full paper by A. Bodor et al.
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder associated to deteriorating motor and cognitive functions, and short survival. The disease is caused by neuronal death which results in progressive muscle wasting and weakness, ultimately leading to lethal respiratory failure. The misbehaviour of a specific protein, TDP-43, which aggregates and becomes toxic in ALS patient’s neurons, is supposed to be one of the causes. TDP-43 is a DNA/RNA-binding protein involved in several functions related to nucleic acid metabolism. Sequestration of TDP-43 aggregates is a possible therapeutic strategy that could alleviate or block pathology. Here, we describe the selection and characterization of a new intracellular antibody (intrabody) against TDP-43 from a llama nanobody library. The structure of the selected intrabody was predicted in silico and the model was used to suggest mutations that enabled to improve its expression yield, facilitating its experimental validation. We showed how coupling experimental methodologies with in silico design may allow us to obtain an antibody able to recognize the RNA binding regions of TDP-43. Our findings illustrate a strategy for the mitigation of TDP-43 proteinopathy in ALS and provide a potential new tool for diagnostics.
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