A strain of Bacillus subtilis (IMD 198) isolated from peat degraded starch to maltose with little production of glucose and other products. Highest levels of enzyme were achieved in a salts medium containing soya bean meal and starch. The enzyme was purified by precipitation with isopropanol, adsorption on calcium phosphate gel and fractionation on DEAE‐and CM‐cellulose ion‐exchange resins. The latter chromatographic procedure removed a contaminating activity that produced dextrins as end‐products from starch or amylose. The action pattern of the purified, major enzyme activity indicates that it may be β‐amylase.
Poly-DL-lactide (PLA) and poly-DL-lactide-co-glycolide (DL-PGA) micromatrices (PharmazomesTM) containing dehydro-isoandrosterone (DHEA), a weak androgen, were prepared by a solvent evaporation process. Micromatrices with increasing drug loading as well as increasing polymer molecular weight were prepared. The morphology of these systems depended on the drug loading, the polymer molecular weight and polymer composition. Increasing the drug loading or polymer molecular weight resulted in increasingly irregular microparticles being formed. DSC thermograms did not reveal the presence of crystalline DHEA in micromatrices containing 10 to 50% DHEA loading. However crystalline DHEA was observed in microspheres heated to above the glass transition temperature of the polymer. X-ray analysis of 30% DHEA micromatrices established the presence of crystalline DHEA in the micromatrices. The percent release of DHEA from the micromatrices, into 40% ethanol at 37OC, increased 1857 Copyright 0 199 I by Marcel Dekker, Inc. Drug Dev Ind Pharm Downloaded from informahealthcare.com by University of Auckland on 11/03/14 For personal use only. Drug Dev Ind Pharm Downloaded from informahealthcare.com by University of Auckland on 11/03/14 For personal use only.
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