A new cationic polythiophene derivative poly[3-(1,1 ′ -dimethyl-4-piperidinemethylene)thiophene-2,5-diyl chloride] (PDPMT-Cl) is synthesized and applied as a colorimetric and fl uorometric probe for the detection of iodide ion and anionic surfactants with high selectivity and sensitivity. Upon adding iodide ion or anionic surfactant into the aqueous solution of PDPMT-Cl, the maximum absorption of the polymer is blue-shifted with a naked-eye color change from red-violet to yellow, signifying that iodide ion or anionic surfactants can cause the dissociation of PDPMT-Cl aggregates. More sensitive results are obtained in the fl uorometric detection to iodide ion or anionic surfactant, thereinto, the detection limit to surfactants can be extended to the order of 10 − 9 M . applied to colorimetric probes for the detection of various bioanalytes. [ 1 − 8 ] However, few of them can be used to detect anionic substrates and the number of colorimetric sensors available at present for anionic substrates remains quite limited. The well-known anionic substrates detection techniques, such as capillary electrophoresis, [ 9 − 15 ] ion chromatography, [ 16 ] normal Raman spectroscopy, [ 17 ] gas chromatography/mass spectrometry (GC-MS), [ 18 ] highperformance liquid chromatography (HPLC), and fl uorescent detection (based on synthetic receptors), [ 19 , 20 ] to some extent, exist some limitations, including time-consuming, requiring expensive equipment, poor selectivity, and sensitivity. Therefore, it is the need of the hour to design new approaches that could improve simplicity, selectivity, and sensitivity.Many systems capable of recognizing, sensing, and transporting negatively charged species are based on supramolecular chemistry. Miyaji et al. [ 21 ] ever reported colorimetric detection of anions by calix[4]pyrrole, but it couldnot monitor the exact concentration, resulting in the limitation of its application. Moreover, though using polymeric membranes doped with various metalloporphyrins or polypyrroles to detect anions were also reported, [ 22 , 23 ] all of them were not still ideal enough to apply. However, E. Li et al.
A lable-free, simple, and sensitive fluorescence ''turn-on'' approach is designed to rapidly detect protein using a conjugated polythiophene derivative (PDPMT-Cl). The fluorescence of PDPMT-Cl solution can be efficiently quenched by PtCl42À ions. Upon adding trypsin to the (bovine serum albumin, BSA) PDPMT-Cl-PtCl42À solution, the BSA is cleaved into amino acid or peptide fragments, which are stronger PtCl42À ions chelators to form more stable complexes with PtCl42À ions. Thus, the PtCl42À ion is displaced from PDPMT-Cl and the fluorescence of PDPMT-Cl is recovered. By triggering the ''turn-on'' signal of PDPMT-Cl, it is successful to detect the protein in real time. ''Turn-on'' response as readout signal is able to effectively reduce background noise and increase detection sensitivity. This method offers good selectivity for detecting protein in the presence of other common amino acids and metal ions. Under optimized conditions, the concentration of BSA in the range of 0.0004-1.75 mg/mL exhibits a linear relationship with the relative fluorescence intensity, and the correlation coefficient is 0.9997. The limit of detection is 4.47 Â 10À4 mg/mL. The system is successfully applied for detecting protein in milk and egg. Due to the simplicity, sensitivity, and rapid response, this assay shows great potential for protein detection in the future. V C 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 939-943, 2013
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