The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated quantities of ribonucleotide reductase. This increase in enzyme activity is due to an increased level of both the M1 and M2 subunit activities. The M1 subunit has been purified from the 600H cell line and shown to consist of a series of six protein spots with apparent molecular weights of 88,000 daltons, but with varying isoelectric points in the range of pH 6.5-7.0. Western blot analyses with antisera against the M1 and M2 proteins indicated that both subunit proteins are present in elevated quantities in the 600H cell line when compared to the wild-type V79 cell line. Southern blot analyses with genomic DNA from the series of stepwise-selected hydroxyurea-resistant cell lines leading to 600H showed that, in latter steps of selection, genomic sequences homologous to a mouse M1 cDNA have undergone a fivefold amplification. This was accompanied by a four- to eightfold increase in the single M1 homologous mRNA.

Abstract. Futures Consciousness refers to the capacity that a person has for understanding, anticipating, and preparing for the future. Although the concept is widely used in the field of futures research, no quantitative tool exists yet that assesses it. Drawing from a recent five-dimensional model that considers Time perspective, Agency beliefs, Openness to alternatives, Systems perception, and Concern for others as interrelated sub-dimensions of a general construct of Futures Consciousness, we developed a composite 20-item scale that measures Futures Consciousness as an interindividual difference. The psychometric properties of this new scale were examined through a dual approach of exploratory and confirmatory factorial analyses with a total of 1,301 participants in three languages (English, French, and Finnish). The scale’s structure proved satisfactory and fitted the hypothesized five-dimensional model in all three languages. Measures of internal and external validity (convergent and concurrent) also indicated good psychometric properties. Notably, individuals’ scores were positively related to the adoption of several social future-oriented behaviors such as pro-environmental and civic behavior. As such, the developed scale proves a reliable tool that could be of use for scholars and practitioners in futures studies as well as psychology.

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The Ecology of Education: Knowledge Systems for Sustainable Development and SustainabilityResearch in knowledge systems for sustainable development (KSSD) seeks to determine how science and technology can be put into effective action at a local level. Teachers in education for sustainability attempt to achieve the same goal. KSSD research has indicated that success is context driven, that panaceas are inappropriate and that knowledge systems at best provide solutions in evolution. In this paper, we describe a teaching framework that we are developing to support KSSD researchers and teachers in education for sustainability based in ecology of education. While a need for ecology in education may be apparent, there is concurrently an equally important need for ecology of education. We argue that one cannot teach ecology in education adequately without an appropriate ecology of education. This paper first explains why teachers need to know this and then describes how teachers of education for sustainability can implement and assess this approach in the classroom.