Objective: The effect of Pimpinella alpina Molk (PaM) in improvement of oxidative stress has been established, but did not for cellular apoptosis. This investigation was conducted to evaluate the effect of PaM on GPx and XO activities, hepatocytes apoptosis and their correlation following UVB radiation. Methods: Forty male rats were assigned into 8 groups: Nor-G, Neg-7, Neg-15, PaM50-7, PaM100-7, PaM150-7, PaM100-15, and PaM150-15. UVB irradiation was given 10 minutes to each rat daily, blood and liver organ samples were taken in day 8 and day 16 to evaluate all variables. Results: Post Hoc statistical analysis showed that GPx activity in PaM150-15 was not significant lower compared to that of Nor-G, p > 0.05. Conversely, the activity of XO in PaM150-7 was not significant higher compared to that of Nor-G, p > 0.05. Expression of Bax and Caspase3 mRNA in PaM100-15 and PaM150 were comparable to that of Nor-G, p > 0.05. The strong negative correlation (-795, p < 0.01) was occur between GPx and XO. Similarly, there were strong negative correlations (-697 and -715, p < 0.001) between GPx and the expression of Bax and Caspase3 mRNA. Conclusion: PaM treatment with 100 -150 mg daily dose for 15 days capable of improving oxidative stress marked by increase in GPx activity and decrease in XO activity and inhibit apoptosis characterized by decrease in the expression of Bax and caspase3 mRNA. There were strong negative correlations between antioxidant activity and apoptosis on Sprague male rats after UVB irradiation.

Ipomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.

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Pendahuluan: Ipomoea batatas L (IPL) mengandung antioksidan betakaroten, vitamin C, dan vitamin E. Rokok mengandung ROS yang terbukti menurunkan kualitas spermatozoa. Tujuan: untuk membuktikan bahwa pemberian IPL dapat memperbaiki jumlah, motilitas, dan viabilitas spermatozoa yang dipapar asap rokok. Metode: Penelitian eksperimental dengan post test only control group design, sebanyak 30 ekor mencit jantan, dibagi menjadi 6 kelompok. Kelompok normal (Nor-G), tidak ada interfensi; Kelompok kontrol negative (Neg-G), hanya dipapar asap rokok; Kelompok kontrol positif (Pos-G), hanya mendapat ekstrak IPL 16 mg/ml; Kelompok IPL-15, IPL-16, dan IPL-17, masing-masing dipapar asap rokok dan mendapat ekstrak IPL 15 mg/ml, 16 mg/ml dan 17 mg/ml.  Hasil: hasil uji Post Hoc LSD menunjukkan bahwa jumlah sperma pada IPL-15, IPL-16, dan IPL-17, lebih tinggi bermakna dibanding Neg-G, p < 0.05 dan lebih rendah dibanding Nor-G dan Pos-G, p < 0.05. Demikian pula dengan persentase viabilitas sperma. Hasil uji Mann Whitney menunjukkan bahwa persentase motilitas sperma pada IPL-15, IPL-16, dan IPL-17, lebih tinggi bermakna dibanding Neg-G, p < 0.05. Sedangkan dibanding Nor-G dan Pos-G persentase motilitas sperma pada IPL-15, IPL-16, dan IPL-17, tidak berbeda bermakna, p > 0.05. Kesimpulan:  pemberian ekstrak ubi jalar jingga dapat meningkatkan jumlah, motilitas, dan viabilitas spermatozoa mencit jantan yang dipapar asap rokok.                       

Introduction: Ipomoea batatas L (LIP) contains antioxidants beta-carotene, vitamin C and vitamin E. Cigarette smoke containing ROS has been proven to decrease sperm quality.Objective: this study aimed to confirm that orange sweet potato extract increases sperm consentration, motility, and viability in mice exposed to cigarette smoke.Methods: In this experimental study with post test only control group design, 30 male mice were divided into 6 groups. Normal group (Nor-G), no intervention, served as the normal control group. Negative control group (Neg-G) was only exposed to cigarette smoke. Positive control group was only treated with IPL 16mg/ml. IPL-15, IPL-16, and IPL-17 group were treated with IPL 15, 16, and 17mg/ml and exposed to cigarette smoke, respectively.Results: Post Hoc LSD analysis showed that means of sperm concentration and viability in IPL-15, IPL-16, and IPL-17 group were significantly higher compared to that of Neg-G (p < 0.05) and were lower compared to that of Nor-G and Pos-G (p < 0.05). Mann Whitney analysis indicated that the persentage of sperm motility in IPL-15, IPL-16, and IPL-17, were significant higher compared to that of Neg-G, p < 0.05. Whilst compared to that of Nor-G and Pos-G the persentage of sperm motility in IPL-15, IPL-16, and IPL-17, there was no significant differences, p > 0.05.Conclusion: treatment of IPL capable of increasing sperm concentration, motility, and viability in male mice were exposed to cigarrette smoke.

Introduction: Pimpinella alpina Molk (PM) is a botanical antioxidant was able to inhibit apoptosis in various cells. Apoptosis is a leading cause of tubular atrophy and therefore chronic kidney disease. However, the effect of PM on reducing apoptosis in kidney cells remains unclear. Objective: aim of this study to elucidate the effect of PM on reducing apoptosis in kidney cells. Methods: In the post test only control group design, 35 male rats were grouped into 7 comprise: NC-G, samples were neither exposure to UVB nor PM treatment; NG-7 and NG-15, all samples were only exposure to UVB irradiation for 7 days; P10-7, P15-7, P10-15, P15-15 groups, samples were exposure to UVB for 7 days and treated with PM for 7 and 15 days respectively. Bax and Caspase3 expression were assessed by rt-PCR and IHC staining method. Results: Statistical analysis showed that RNA-Bax and RNA-caspase3, Bax and caspase3 protein expression in P15-7, P10-15 and P15-15 were lower significantly compared to those of NG-7, p<0.05, and no significant difference compared to those of NC-G, p > 0.05. Conclusion: PM treatment with 100 and 150 mg/day for seven and fifteen days were able to decrease Bax and Caspase3 expression in kidney cells following UVB irradiation. Even, the decreased in Bax and caspase3 expression were comparable to normal.

Objective: The effect of Pimpinela alpina Molk (PaM) on decrease in Bax and Caspase-3 protein expression in liver cells apoptosis have been proven. However, the difference result between 7 and 15 days treatment duration of PaM need to be confirmed. This study aimed to confirm that treatment of PaM during 15 days is more effective decreasing Bax and Caspase-3 protein expression in liver cells following UVB irradiation. Methods: In the post test only control group design, 35 Sprague Dawley male rats, 300 gram body weight were divided into two arms, consisting of three groups respectively. First arm comprise Neg-7, PaM7-100, and PaM7-150. Second arm comprise Neg-15, PaM15-100, and PaM15-150. Nor-G was added as normal control neither exposed to UVB nor PaM treatment. In negative group was only radiated to UVB and PaM groups were exposed to UVB and treatment with 100, and 150 mg PaM per oral for 7 and 15 days respectively. At day 8 (first arm) and 16 (second arm), liver organ was taken and Bax and Caspase-3 protein expression assessed by Immunohistochemical staining method. Result: Post Hoc LSD analysis indicated that Bax and Caspase-3 protein expression in PaM15-100 and PaM15-150 was significant lower compared to that of Nor-G, PaM7-100, and PaM7-150, p < 0.05. Conclusion: Ttreatment of PaM with doses 100 and 150 mg for 15 days was better in decreasing Bax and Caspase-3 protein expression of liver cells following UVB irradiation. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.296-303

Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.